摘要
采用PCR-SSCP技术,对五指山猪、海南地方猪2个品系(临高猪、屯昌猪)等猪种的IGFBP6基因的多态性进行研究。结果表明,引物P1和P2的PCR片断SSCP分析表现有多态性,且出现3种基因型。随后的测序结果表明,它们都在相应的区域存在着变异;引物P1在海南地方猪种大多表现BB基因型和以B等位基因为主,引物P2大多表现CC基因型和以C等位基因为主。SPSS软件分析五指山猪IGFBP6基因多态性与其3个生长阶段的关联程度,结果发现,引物P1 AA基因型和引物P2 DD基因型的猪个体在2月龄、8月龄、12月龄的生长体重高于其它基因型,但个体差异均不显著(P>0.05)。
The PCR-SSCP in IGFBP6 gene and their correlations with growth traits were investigated in Hainan WZS pig in a closed breeding colony, in Hainan black pigs （Lingao pig and Tunchang pig）. The results showed there were two alleles and three genotypes in PCR products of IGFBP6 gene.Subsequent sequencing results showed that they were all there was variation in the corresponding area.The result of population genetics analysis, showed that in the mutation of P1, the frequency of BB genotype were significantly higher than AA genotype, re-spectively.In the mutation of primer P2, the frequency of CC genotype were higher than other genotypes. The least square analysis showed that the body weight of 2, 8 and 12-month-old pigs were higher than other month pigs ,but there was no significance（P〉0.05）, respectively.
出处
《养猪》
2015年第2期70-72,共3页
Swine Production
基金
海南省自然科学基金项目(313098)
海南省自然科学基金项目(312080)
海南省引进集成应用专项(YJJC20120009)
