摘要
研究利用Sephadex G-200凝胶柱层析法和纤维素DE-52离子交换柱层析法分离纯化经过Saio法提取的大豆7S球蛋白样品,采用SDS-聚丙烯酰胺凝胶电泳和高效毛细管电泳进一步鉴定Saio法提取的大豆7S球蛋白样品的组分及含量。结果表明,Saio法提取的大豆7S球蛋白纯度约为81%。
The soybean 7S globulin extracted by Saio method was separated and purified by the methods of Sephadex G-200 gel chromatography and cellulose DE-52 ion-exchange chromatography,and its component and content were further identified by SDS-PAGE and high performance capillary electrophoresis.The results showed that the purity of soybean 7S globulin extracted by Saio method was about 81%.
出处
《中国油脂》
CAS
CSCD
北大核心
2015年第4期14-17,共4页
China Oils and Fats
基金
国家863项目(2013AA102206)
国家自然科学基金项目(31301427)
关键词
大豆
7S球蛋白
纯化
鉴定
soybean
7S globulin
purification
identification
