摘要
目的:利用紫外-可见光谱和薄层色谱对黄芩茎叶进行鉴别。方法:采用紫外-可见光谱法分别对石油醚、氯仿、无水乙醇、水4种溶剂的黄芩茎叶浸出液进行紫外-可见光谱扫描,并测定其吸收峰数目和其峰位置;采用聚酰胺和硅胶G作为吸附剂对黄芩茎叶进行薄层展开,观察其成分的分离情况。结果:紫外-可见光谱中不同提取溶剂提取同一种样品时,所得的吸收峰数目及峰位置都有差异;薄层色谱中采用不同的吸附剂和展开剂,均能使黄芩成分得到较好分离。结论:这两种方法简便、准确、灵敏,可用于黄芩茎叶的快速鉴别。
Objective：To identify stems and leaves of Scutellaria baicalensis georgi by ultraviolet-visible spectroscopy（UV- visible spectra） and thin-layer chromatography（TLC）. Methods：The extracts of petroleum ether,chloroform,ethanol and water of stems and leaves of Scutellaria baicalensis georgi were scanned by UV-visible spectra respectively. The number and position of the absorption peaks were also determined. With polyamide and silica gel G as adsorbent,the TLC of stems and leaves of Scutellaria baicalensis georgi was done and the separation of its components were observed. Results：In UV-visible spectra,extracting same sample from different extraction solvent can get different number and position of absorption peaks;in TLC,all different adsorbents and developing solvents can make a good separation of components of Scutellaria baicalensis georgi. Conclusion：Both the two methods are simple,accurate and sensitive,so they can be used for rapid identification of stems and leaves of Scutellaria baicalensis georgi.
出处
《山东中医杂志》
2015年第4期284-286,共3页
Shandong Journal of Traditional Chinese Medicine
基金
山东省自然科学基金资助项目(编号:ZR2009CQ025)
关键词
黄芩茎叶
紫外-可见光谱
薄层色谱
stems and leaves of Scutellaria baicalensis georgi
ultraviolet-visible spectroscopy（UV-visible spectra）
thin-layer chromatography（TLC）
