摘要
目的探讨水飞蓟宾对人乳腺癌细胞生长和侵袭转移的影响及机制。方法用不同浓度水飞蓟宾处理人乳腺癌细胞系MCF-7 24h,采用MTT(噻唑蓝)法和台盼蓝染色法检测水飞蓟宾对MCF-7细胞的抑制作用;采用transwell技术检测水飞蓟宾对MCF-7细胞侵袭转移的影响;Western blot检测水飞蓟宾对MCF-7细胞MEK及ERK(均为丝裂原激活的蛋白激酶)蛋白磷酸化水平及基质金属蛋白酶-9(MMP-9)表达水平的影响。结果水飞蓟宾可显著抑制MCF-7细胞的增殖并造成肿瘤细胞死亡,与对照组比较,水飞蓟宾增殖抑制率[20μM(8.9±3.8)%、50μM(19.7±4.2)%、100μM(29.8±5.7)%比0]、台盼蓝染色率[50μM(16.1±4.8)%、100μM(25.3±5.1)%比(3.4±1.4)%],均升高(P<0.05,P<0.01);水飞蓟宾通过抑制MEK(水飞蓟宾20μM:0.26±0.03、50μM:0.15±0.03、100μM:0.11±0.02比对照组:0.35±0.04)和ERK(水飞蓟宾20μM:0.23±0.03、50μM:0.12±0.02、100μM:0.07±0.01比对照组:0.32±0.05)的磷酸化降低MMP-9(水飞蓟宾50μM:0.18±0.03、100μM:0.14±0.02比对照组:0.29±0.04)的表达并抑制MCF-7[水飞蓟宾20μM:(14.3±2.1)%、50μM:(35.6±5.7)%、100μM(53.1±6.5)%比对照组:0]细胞侵袭转移的能力(P<0.05,P<0.01)。结论水飞蓟宾抑制人乳腺癌细胞的生长和侵袭转移。
Objective To investigate the effect of silibinin on proliferation and invasion of breast cancer cells and the underlying mechanism. Methods Human breast cancer cell line MCF-7 were treated with various concen-trations of silibinin for 24h, then MTT assay was used to detect cell proliferation. The effect of silibinin on inva-sion of MCF-7 cells was measured by transwell. The phosphorylation of MEK and ERK proteins and the expression of MM-9 protein were evaluated by western blot. Results The inhibitory rate of silibinin on MCF-7 cell prolifer-ation was （8.9±3.8）% at 20μM, （19.7±4.2）% at 50 μM, and （29.8±5.7）% at 100μM, with significant difference compared with control group（P〈0.05）. Typan blue staining rate of MCF-7 cells treated with silibinin at concentration of 50 and 100μM was （16.1±4.8）% and （25.3±5.1）%, respectively, both with significant difference compared with that of control group [（3.4±1.4）%, P〈0.05]. Silibinin at concentration of 20, 50, and 100μM inhibited the phospho-rylation of MEK （0.26±0.03, 0.15±0.03, 0.11±0.02 vs 0.35±0.04） and ERK （0.23±0.03, 0.12±0.02, 0.07±0.01 vs 0.32±0.05） proteins. Silibinin of 50 and 100μM decreased the expression of MMP-9 protein （0.18±0.03, 0.14±0.02 vs 0.29±0.04）. Silibinin inhibited metastasis and invasion of MCF-7 cells[20μM：（14.3±2.1%）, 50μM：（35.6±5.7）%, 100μM：（53.1±6.5）% vs 0]. Conclusion Silibinin can inhibit the growth and invasion of breast cancer cells.
出处
《浙江中西医结合杂志》
2015年第4期337-340,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
关键词
乳腺癌
水飞蓟宾
MMP-9
肿瘤侵袭
MEK/ERK
Breat cancer
Silibinin
MEK/ERK
MMP-9
Tumor invasion
