摘要
[目的]应用微核实验和彗星电泳实验评价氯化镧亚慢性染毒致小鼠遗传毒性作用。[方法]将100只SPF级ICR小鼠随机分为5组:4个氯化镧染毒组和1个对照组,每组20只,雌雄各半。5个剂量组分别以灌胃的方式给予不同浓度的氯化镧溶液(0、10、20、50和100 mg/kg)。每周染毒6次,连续染毒13周后,取小鼠外周血进行彗星实验及镧含量的测定,取小鼠骨髓观察骨髓细胞微核率。[结果]镧染毒剂量达50 mg/kg时,彗星细胞的尾长及尾部DNA含量与对照组相比,差异有统计学意义(P<0.05),小鼠骨髓细胞微核率与对照组相比,差异有统计学意义(P<0.05)。4个氯化镧染毒组血液中均有不同程度的镧的蓄积,与对照组相比,差异均有统计学意义(P<0.05)。[结论]稀土镧元素可以在血液中蓄积,具有一定的遗传毒性。
[ Objective ] To evaluate the genotoxicity of sub-chronic exposure to lanthanum chloride on mice using micronucleus test and comet assay. [ Methods ] SPF grade ICR mice (n=100) were randomly divided into five groups, including four lanthanum chloride exposure groups and a control group, 20 mice in each group, male and female half and half. The five dose groups were fed by gavage respectively with different concentrations of lanthanum chloride solution (0, 10, 20, 50, and 100 mg/kg), 6 times a week, for 13 conseentive weeks. Peripheral blood samples of mice were collected for comet assay to determine lanthanum content, and bone marrow samples were taken to observe bone marrow micronucleus rate. [ Results ] The comet tail length of the ceils and the tail DNA content in the groups exposed to lanthanum 50 mg/kg and up were significantly different compared with the control group (P〈 0.05), so were mouse bone marrow micronucleus rates (P 〈 0.05). The four lanthanum chloride treated groups showed varied degrees of lanthanum accumulation in blood, and showed significant differences compared with the control group (P 〈 0.05). [ Conclusion ] Lanthanum could accumulate in blood and lead to genetic toxicity.
出处
《环境与职业医学》
CAS
CSCD
北大核心
2015年第4期351-354,共4页
Journal of Environmental and Occupational Medicine
基金
内蒙古教育厅资助项目(编号:NJZY12227)
关键词
氯化镧
遗传毒性
微核实验
彗星实验
蓄积量
小鼠
lanthanum chloride
genetic toxicity
micronucleus test
comet assay
accumulation
mouse