摘要
目的探讨miR-200c对人舌鳞状细胞癌Tca8113细胞增殖和凋亡的影响。方法将miR-200c的模拟物通过脂质体转染到人舌鳞状细胞癌Tca8113细胞内,通过四甲基偶氮唑盐(MTT)法检测细胞增殖能力、流式细胞术检测细胞周期和细胞凋亡率、Western blot检测半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)和Bcl-2蛋白的表达。结果与对照组相比,miR-200c模拟物20、40、80nmol/L组的Tca8113细胞生长受到明显抑制,差异有统计学意义(P<0.05),miR-200c模拟物的浓度越大,作用时间越长,抑制效果越明显(P<0.05);转染miR-200c模拟物48h后,Tca8113细胞停滞于G0/G1期,细胞凋亡率显著增加,差异有统计学意义(P<0.05);Western blot证明20、40、80nmol/L组Bcl-2蛋白表达量明显低于对照组,而Caspase-3蛋白表达量明显高于对照组(P<0.05)。结论 miR-200c过表达可抑制舌癌Tca8113细胞增殖并诱导细胞凋亡。
Objective To investigate the effects of miR-200 con proliferation and apoptosis of tongue squamous cell carcinoma（TCCS）Tca8113cells.Methods The mimics of miR-200 cwere transfected into Tca8113 cells using liposome.The Tca8113 cell proliferation was detected by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay.The flow cytometry assay was used to determine the cell cycle and the apoptosis rate of Tca8113 cell.The protein expression levels of Bcl-2and Caspase-3in Tca8113 cell was detected by Western-blot.Results The 20,40,80nmol/L miR-200 cmimics groups inhibited the growth of Tca8113 cells,the difference compared with the control group showing statistical significance（P〈0.05）.The greater the miR-200 c mimics concentration and the longer duration of action,the more significant the inhibition effect（P〈0.05）.After 48 htransfecting by miR-200 cmimics,the Tca8113 cells were arrested in the G0/G1 phases of cell cycle,and the apoptosis rate of the miR-200 cmimics groups was significantly increased,the difference compared with the control group showing statistical significance（P〈0.05）;Western blot verified that the expression amount of Bcl-2protein in the 20,40,80nmol/L miR-200 cgroups was significantly lower than that in the control group,while the expression amount of Caspase-3protein was significantly higher than that in the control group（P〈0.05）.Conclusion The overexpression of miR-200 cmight inhibit the proliferation of Tca8113 cell and induces their apoptosis.
出处
《重庆医学》
CAS
北大核心
2015年第10期1322-1324,共3页
Chongqing medicine
基金
广西高等学校科研资助项目(201204LX329)
右江民族医学院科研资助项目[右医科字(2011)1号]
