摘要
目的:探讨丹红注射液对大鼠单侧睾丸扭转复位后健侧睾丸的组织学以及附睾精子生成的影响。方法:将30只健康雄性Wi st ar大鼠随机分为假手术组(A组)、睾丸扭转复位组(B组)、丹红注射液处理组(C组),每组10只。按Tur ner法建立左侧睾丸扭转模型,A组大鼠切开左侧阴囊,使左侧睾丸充分游离暴露,不予以扭转,模拟Tur ner法手术过程随意搬动睾丸,随后缝合关闭阴囊;B组大鼠按Tur ner法制作睾丸扭转模型:左侧睾丸顺时针绕精索旋转720°,肉膜白膜缝合固定以防止复位,阴囊缝合,扭转6小时后复位固定;C组大鼠按上述相同方法制备睾丸扭转模型,于睾丸扭转复位前60分钟腹腔注射丹红注射液(2 m L/kg),同时在术后3天每天分别注射丹红注射液(2 m L/kg)。各组喂养至术后4周时处死,取右侧睾丸标本行组织病理学观察,同时取右侧附睾尾部行精子计数及活动率检测。检测睾丸组织一氧化氮合酶(NOS)、血清超氧化物歧化酶(SOD)和丙二醛(MDA)水平。结果:C组与B组相比,生精上皮层数增多,平均曲细精管直径(MSTD)增大,同时附睾精子计数与活动率明显升高,其差异有统计学意义(P<0.05)。C组大鼠睾丸组织SOD水平升高,NOS、MDA水平降低,差异有统计学意义(P<0.05)。结论:丹红注射液能够减轻大鼠单侧睾丸扭转复位后健侧睾丸组织损害,使附睾精子生成显著增多,机制可能为减少大鼠睾丸组织内自由基生成,提高其抗自由基能力。
Objective：To study the effect of DanHong injection on the histology of healthy testis and the sperm production of epididymides of rats undergoing the reduction by testicular torsion on the affected side. Methods：The 30 male Wistar rats were randomized into the sham-operated group （A）, the reduction group （B） and the injection group （C） with 10 rats in each. The models with testicular torsion established by Turner method, rats in group A were cut the left scrotum to isolate and expose the testis without torsion and rotate the testis by imitating the Turner operating process and lastly stitched the wound; as in the a group the models established, the left testis of rats in group B were rotated 720°clockwise round the varicosity to be fixed by stitching the sarcolemma and albuginea and the left scrotum were closed, after fixation for 6 hours the testis were reduced and fixed;the models established as in the A and B group, the testis in the C group were injected DanHong injection 2 mL/kg in the abdomen before the re-duction of torsion for 60 minutes and after the operation the rats were injected DanHong injection 2 mL/kg for 3 days. After feeding for 4 weeks, the rats of three groups were killed, the right testis were collected to be observed histopathologically, the sperm of the right epididymides were counted and their activity ratio were detected. Mean-while, the levels of the nitricoxide synthase （NOS）, serum superoxide dismutases （SOD） and malondialdehyde （MDA） were determined respectively. Results：In the comparison of the C and B group, the quantities of seminifer-ous epithelium cell of the rats in group C were increased, the diameters of the seminiferous tubules were enlarged, sperm counts of epididymides and activity ratios were raised significantly, and the differences between the two groups were significant （P〈0.05）. The SOD levels of the rats＇ testes in group C were increased, while the NOS and MDA levels were decreased, and the differences showed statistical meaning
出处
《西部中医药》
2015年第2期15-17,共3页
Western Journal of Traditional Chinese Medicine
