摘要
在使用微流控芯片的背景下,对自然界切换系统的研究及单个生物实体的研究代表了数字生物化学的重要内容。综述了以微流控芯片为特征的数字生物化学在以上2个领域的进展。提出微流控芯片技术促进了对切换系统的分析,且切换系统可提供"模数"信号转换的新手段,使其应用于微流控检测系统上;微流控分配技术可以分配和研究单个生物实体,对于细胞内生物过程的重建和研究很有意义,还可以进行单个生物实体之间相互作用的研究,考察分子群、细胞或生物体的内在异质性;该系统因更容易产生切换,故增强了对细胞和生物分子的灵敏度,且因"数字化"是通过定性的测定来提供定量信息,故其在耐用性、实验设计的灵活性和简便性方面比传统方法更具优势。该微流体数字化技术未来会被应用于更多新的生物化学测定中。
Using microfluidics as a frame of reference, two areas of research are identified within digital biochemistry： the study of natural switching systems and the study of single biological entities.The development of these two areas were reviewed.h is pointed out that microfluidics accelerates analysis of switching systems, Conversely, such switching systems can be used to create new kinds of microfluidic detection systems that provide ＂analog-to-digital＂ signal conversion; microfluidic compartmentalization technologies for studying and isolating single entities can be used to reconstruct and understand cellular processes, study interactions between single biological entities; investigate the inner heterogeneity of molecular group, cell or organism ; compartmentalization of single entities can also be used to induce switching in systems that do not naturally switch, as a result, the sensitivity of detecting biomolecules was greatly enhanced,this ＂digitizing＂ offers advantages in terms of robustness, assay design, and simplicity because quantitative information can be obtained with qualitative measurements.h is anticipated that in the future they will enable quantitative measurements.
出处
《煤炭与化工》
CAS
2015年第2期114-117,共4页
Coal and Chemical Industry
关键词
微流控芯片
数字化
生物化学
切换系统
分配技术
microfluidic chip
digital
biochemistry
switching system
compartmentalization new chemistries to be used for
