摘要
目的:探讨阳和汤对裸鼠荷人乳腺癌组织中CD90表达及其抑瘤作用。方法:将人乳腺癌细胞培养接种于裸鼠第四乳腺脂肪垫,建立裸鼠荷人乳腺癌模型,随机将荷瘤裸鼠分为5组:空白对照组、环磷酰胺组、阳和汤低浓度组、阳和汤中浓度组、阳和汤高浓度组,并于接种后14 d给药2周,给药结束后24 h麻醉,拉颈处死裸鼠,剥离肿瘤组织,称取瘤质量并绘制瘤体生长曲线、计算抑瘤率及采用免疫组化法测定组织中CD90的表达。结果:1)环磷酰胺组及阳和汤组CD90表达均低于空白对照组,差异有统计学意义(P<0.05),而环磷酰胺组与阳和汤组比较差异无统计学意义(P>0.05),环磷酰胺组、阳和汤低浓度组、阳和汤中浓度组、阳和汤高浓度组的表达阳性率均数分别为0.34%、0.73%、0.52%及0.42%;2)环磷酰胺组及阳和汤组肿瘤体积及瘤质量均小于空白对照组,差异有统计学意义(P<0.05),而环磷酰胺组与阳和汤组比较差异无统计学意义(P>0.05),环磷酰胺组、阳和汤低浓度组、阳和汤中浓度组、阳和汤高浓度组的抑瘤率分别为63.64%、37.45%、47.64%及63.27%。结论:1)阳和汤对人乳腺癌组织中CD90表达具有明显下调作用,可能是其抑制乳腺癌的作用机制之一;2)对人乳腺癌细胞的生长具有明显抑制作用。
Objective： To explore the effect of Yanghe Decoction on the expression of CD90 and its antitumor effect in nude mice bearing human breast cancer. Methods： The breast cancer cells were injected into the fourth mammary fat pads of nude mice,establishing the model of nude mice bearing human breast cancer. Then the mice were randomly divided into 5 groups： blank control group,cyclophosphamide group,Yanghe Decoction low dose group,middle dose group and high dose group. After 14 days inoculation,model mice were administered,tumors were collected after 2 weeks. Tumor weight and expression of CD90 were measured.Results： With regards to expression of CD90 and tumor volume and weight,cyclophosphamide group and Yanghe all dose groups were statistically lower than those of the blank control group（ P 0. 05）,but there was no significant differences between the cyclophosphamide group and Yanghe dose groups（ P 0. 05）. The positive expression rate of cyclophosphamide group,low,middle,high dose groups were 0. 34%,0. 73%,0. 52% and 0. 42%; the tumor inhibition rates were 63. 64%,37. 45%,47. 64%and 63. 27%. Conclusion： Yanghe Decoction can obviously inhibit the growth of human breast cancer cells and down-regulate CD90 in human breast cancer tissue,which may be one of the mechanisms of breast cancer inhibition.
出处
《世界中医药》
CAS
2015年第3期391-393,398,共4页
World Chinese Medicine
基金
国家自然科学基金项目(编号:81473669)
四川省科技厅项目(编号:2014JY0243)
关键词
乳腺癌
阳和汤
荷瘤裸鼠模型
Breast cancer
Yang He Decoction
Nude mice model
