摘要
建立了加压毛细管电色谱法测定抗真菌药米卡芬净。采用EP-100-20/45-3-C18-BP反相毛细管色谱柱,以乙腈∶2.5 mmol/L磷酸盐缓冲液（p H 6.5）（35∶65）为流动相,检测波长270 nm,分离电压15 k V。米卡芬净在62.5~800μg/ml浓度范围内线性关系良好,平均回收率为99.6%,RSD为1.47%。
A pressurized capillary electrochromatogrphy（p CEC） method was established for the determination of antifungal antibiotic micafungin. An EP-100-20/45-3-C18-BP reverse phase capillary chromatographic column was used, with the mobile phase of acetonitrile∶2.5 mmol/L phosphate buffer（p H 6.5）（35∶65）, at the detection wavelength of 270 nm and separation voltage of 15 k V. It was linear in the range of 62.5- 800 ug/ml. The average recovery was 99.6%, with RSD of 1.47%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2015年第4期378-380,共3页
Chinese Journal of Pharmaceuticals
基金
国家重大科学仪器设备开发专项(2011YQ15007208)
国家重大专项(2014zx09201001-012)
国家"重大新药创新"科技重大专项(2012ZX09301002-003)
关键词
米卡芬净
抗真菌药
加压毛细管电色谱
测定
micafungin
antifungal drug
pressurized capillary electrochromatography
determination
