摘要
目的:研究乙醇体外诱导人脐静脉内皮细胞(HUVEC)凋亡,及对细胞间黏附分子(ICAM-1)蛋白表达的影响。方法:以不同浓度乙醇(50、100和200mmol/L)作用于体外培养的人脐静脉内皮细胞24h,四甲基偶氮唑盐(MTT)比色法测定各组细胞活力;流式细胞仪检测细胞凋亡率;酶联免疫吸附法检测ICAM-1蛋白表达。结果:乙醇能抑制内皮细胞活力,促进细胞凋亡,增加ICAM-1表达,并呈浓度依赖性。结论:乙醇能诱导内皮细胞损伤,其机制可能与促进细胞凋亡、影响ICAM-1蛋白表达有关。
AIM：To study the apoptosis induced by ethanol on human umbilical vein endothelial cells（HUVEC）and the expression of ICAM-1 in vitro. METHODS： HUVEC were treated with different concentrations of ethanol（50,100 and 200mmol/L）for 24 hours,the activity of cells was determined by methyl thiazolyl tetrazolium（MTT）assay;the rate of apoptosis was detected by flow cytometry（FCM）assay;and the expression of ICAM-1by enzyme linked immunosorbent assay.RESULTS：Ethanol can inhibit the cells activity,promote the apoptosis,increase the expression of ICAM-1of HUVEC in dose-dependent manner.CONCLUSION：Ethanol can damage endothelial cells and its possible mechanism may be linked to promote the apoptosis and the expression of ICAM-1.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2015年第3期266-268,308,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
