摘要
目的探讨氨基甲酸乙酯对正常小鼠免疫功能的影响,为制订食品安全标准提供实验依据。方法设氨基甲酸乙酯0.17、0.83和1.67 mg/kg·bw 3个剂量组,同时设阴性对照组(蒸馏水),从细胞免疫、体液免疫、单核巨噬细胞吞噬作用及自然杀伤细胞的攻击作用和免疫器官的脏体比五方面观察氨基甲酸乙酯对正常小鼠免疫功能的影响。结果与阴性对照组比较,氨基甲酸乙酯1.67 mg/kg·bw剂量组能明显抑制Con A诱导的小鼠脾淋巴细胞的增殖能力、NK细胞活性和降低溶血空斑数(P<0.05);氨基甲酸乙酯3个剂量组对小鼠单核巨噬细胞吞噬功能和胸腺/体重、脾脏/体重比值均无明显影响(P>0.05)。结论氨基甲酸乙酯对正常小鼠免疫功能具有一定的抑制作用。
Objective To evaluate the effects of low dose Ethyl Carbamate（ EC） on the immune function of ICR mice and to provide evidences for developing food safety standard. Methods The ICR mice were divided into four groups,and three groups were treated with 0. 17,0. 83,1. 67 mg / kg·bw EC respectively and the control group was treated with distilled water only. The immune function of ICR mice was determined by five aspects,including cellular immunity,humoral immunity,mononuclear macrophages＇ s phagocytosis,natural killer cell activity and the organ coefficients of immune organs.Results Compared with the control group,the 1. 67 mg / kg · bw EC significantly inhibited the proliferation of spleen lymphocyte,natural killer cell activity and the hemolysis plaque- forming ability induced by Con A（ P〈0. 05）.Conclusion EC can cause the inhibition of normal mouse＇s immune function.
出处
《浙江预防医学》
2015年第4期362-365,共4页
Zhejiang Journal of Preventive Medicine
基金
国家食品安全标准制定项目(spaq-2011-54)
