摘要
研究了复合纤维素酶对漂白马尾松纤维表面形貌、形态参数和结构的影响.实验结果表明,在一定范围内,增加反应时间和酶用量都可以增强酶水解的反应程度.酶优先作用于纤维表面和样品中本身存在的细小纤维.随着酶用量的增加,纤维逐渐发生起皮、剥皮、断裂等现象,细小纤维含量逐渐增加,纤维的平均长度先略微增加后逐渐降低,其平均聚合度和反应效率在逐渐下降.当酶用量为20U·g-1时,纤维的平均长度下降70%,细小纤维含量由16.8%增加至72.4%,聚合度下降53%.结晶度分析表明,复合纤维素酶首先主要作用于纤维素的无定形区,随着酶用量的增加,酶同时水解结晶区和无定形区,结晶度呈现先增大后减小的周期性变化,但仍保持典型的纤维素I的结构.
The effect of complex cellulase on the bleaching masson pine fiber surface,morphology parameter and structure was investigated.The results showed that increasing the time and dosage can enhance the degree of hydrolysis reaction within a certain range.The cellulase preferentially acted on fines existed in the sample and on the surface of the fiber.With the increasing of dosage,peeling and breaking occured,fines content increased,the average length increased at first and then gradually reduced.The degree of polymerization of the cellulase and reaction efficiency was gradually decreased.When the dosage was 20 U·g-1,the average length decreased by 70%,fines content increased from 16.8%to 72.4%,the degree of polymerization decreased by 53%.The crystallinity had a periodical change which increased first and then decreased.Within a certain time,the crystallinity showed that cellulase hydrolysed the amorphous at first,then cellulase acted on the amorphous and crystallization zone at the same time,but the crystal structure remained the typical cellulose I.
出处
《陕西科技大学学报（自然科学版）》
2015年第2期1-6,18,共7页
Journal of Shaanxi University of Science & Technology
基金
国家自然科学基金项目(31370578)
陕西省科技厅自然科学基础研究计划项目(2014JZ013)
陕西省科技厅重点实验室科研计划项目(2011HBSZS014)
陕西科技大学博士科研启动基金项目(BJ13-21)
关键词
复合纤维素酶
酶水解
纤维形态
结晶度
聚合度
complex cellulase
enzymatic hydrolysis
fiber morphology
crystallinity
degree of polymerization
