摘要
目的探索鸡和疣鼻栖鸭Ia-相关的恒定链(CIi和MDIi)的交叉免疫原性。方法用间接ELISA检测抗CIi小鼠血清与原核表达MDIi的反应性、抗MDIi小鼠血清与原核表达CIi的反应性;通过荧光免疫组织化学法鉴定抗CIi小鼠血清与疣鼻栖鸭脾组织MDIi的荧光反应强度、抗MDIi小鼠血清与鸡肝组织CIi的荧光反应强度。结果抗MDIi小鼠血清与原核表达CIi的反应滴度为1∶8000,抗CIi小鼠血清与原核表达MDIi的反应滴度为1∶16 000;采用抗MDIi小鼠血清、抗CIi小鼠血清进行免疫荧光组织化学染色,均能检测异种鸟类组织内的Ii抗原,但荧光染色强度均弱于同种鸟类Ii抗原组织的荧光反应强度。结论 CIi和MDIi具有交叉免疫原性,CIi和MDIi的序列保守性是形成共同抗原表位的分子基础,从免疫学角度揭示了鸟类Ii的高度保守性。
Objective To explore the cross immunogenicity between Ia-associated invariant chain（Ii） of chicken（CIi）and Ii of Muscovy duck（MDIi）.Methods The immunoreactivity between mouse anti-CIi serum and the MDIi expressed by prokaryotic cell,and the immunoreactivity between the mouse anti-MDIi serum and the CIi expressed by prokaryotic cell were detected through indirect ELISA.To detect the immunoreactivity between the mouse anti-CIi serum and the MDIi of the spleen tissues of Muscovy duck,and the immunoreactivity between the mouse anti-MDIi serum and the CIi of the liver tissues of chicken（tissular CIi）,the fluorescence immunohistochemistry assay was performed using Dy Light 488 affini Pure goat anti-mouse Ig G as the second antibody.The fluorescence intensity was detected in each tissue by Leica fluorescence microscopic imaging system.Results The titer of ELISA reactivity between mouse anti-MDIi serum and the expressed CIi was 1∶8000.The titer of ELISA reactivity between mouse anti-CIi serum and the expressed MDIi was 1∶16 000.The mouse anti-CIi serum and anti-MDIi serum could respectively react with tissular MDIi and tissular CIi,showing visible green fluorescence in corresponding cells of each tissue.But the fluorescence intensities in the above tissues were weaker than those in positive control tissues in which the mouse anti-MDIi serum or anti-CIi serum reacted with tissular MDIi or tissular CIi respectively.Conclusion The cross immunogenicity between CIi and MDIi has been verified through the immunological experiments.The molecular basis of common antigen epitope formation between CIi and MDIi involves the conservation of the two Ii sequences,which reveals the high conservative property of Ii among variant birds from the perspective of immunology.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第4期462-466,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
安徽省高校自然科学研究重点项目(KJ2013A203)
国家自然科学基金应急管理项目(31440025)
安徽省教育厅特色专业重点项目(2014tszy092)
安徽省省级学科建设重大项目(皖教秘科[2014]28号)
关键词
疣鼻栖鸭
鸡
恒定链
ELISA
荧光免疫组织化学
共同抗原
muscovy duck（Cairina moschata）
chicken（Gallus domestiaus）
Ia-associated invariant chain（Ii）
ELISA
fluorescence immunohistochemistry
common antigen
