摘要
环介导等温扩增法(LAMP)是近年发展起来的新型核酸检测技术,因其检测特异性好、灵敏度高、时间短、无需热循环设备而在病原体检测中得到广泛应用。基于该技术进行的改造和改良也层出不穷,本文从该技术的方法改造、缩短反应时间和简化模板预处理、多重扩增产物分析、防止假阳性污染四个方面对近几年LAMP技术的发展进行综述,为今后以该技术平台为基础的创新和应用提供理论依据。
The loop-mediated isothermal amplification（LAMP） technique has been widely applied in the detection of nucleic acid because of its high specificity and sensitivity, time efficiency, and independent of thermo cycling equipment. After the invention of LAMP many studies have been done to modify and improve this method. This article has summarized these studies and reviewed the recent developments of LAMP technology from the following four aspects： methodology improvements; Shortening of reaction time and simplification of templates preprocessing; multiple products analysis; inhibition of false positive pollution. The objective of this review is to provide the theoretical foundation for the development of future novel nucleic acid detection platforms which will based on the LAMP technique.
出处
《微生物学通报》
CAS
CSCD
北大核心
2015年第4期729-735,共7页
Microbiology China
基金
南京军区南总医院院内课题(No.2013053)
关键词
环介导等温扩增
原理
多重产物分析
Loop-mediated isothermal amplification
Methodology
Multiple products analysis
