摘要
目的研究肾脏淀粉样变性病患者α1-抗胰蛋白酶和蛋白溶解酶-9的表达特点,探讨其在本病进展中的作用。方法 2006年1月至2014年1月于黄石市中心医院肾内科治疗或诊断的AL型肾脏淀粉样变性病患者共60例(其中蛋白尿期42、肾病综合征期16例、尿毒症期2例),比较蛋白尿期与肾病综合征期患者血清肾功能,同时对两组肾脏切片做免疫组化观察,最后ELISA与Western blot法测定两组肾脏提取液中α1-抗胰蛋白酶与蛋白溶解酶-9表达,RT-PCR法测定mRNA表达。结果肾病综合征期患者血清血尿素氮、血肌酐、血尿素以及血尿酸明显高于蛋白尿期患者(P<0.05);肾脏切片免疫组化示,α1-抗胰蛋白酶与蛋白溶解酶-9 2个指标肾病综合征期患者明显少于蛋白尿期患者(P<0.05);ELISA以及Western blot显示α1-抗胰蛋白酶与蛋白溶解酶-9表达肾病综合征期患者均明显低于蛋白尿期患者(P<0.05),RT-PCR结果显示,α1-抗胰蛋白酶与蛋白溶解酶-9 mRNA表达肾病综合征期患者明显低于蛋白尿期患者(P<0.05)。结论α1-抗胰蛋白酶和蛋白溶解酶-9与AL型肾脏淀粉样变性病的发生与发展密切相关,今后或可作为一个治疗靶点。
Objective To study the expression characteristics of the α1-antitrypsin and matrix metalloprotein-9 of renal amyloidosis patients, and explore theirrole in the progress of the disease. Methods 60 cases under treatment or diagnosis of AL type renal amyloidosis in renal Department of internal medicine in our hospital from 2006 January to2014 January were recruited for the study（including proteinuria in period 42, period 16 cases of nephrotic syndrome, 2patients with uremia）. Serum renal function of the patients with proteinuria and nephrotic syndrome patients were compared. Immunohistochemical analyzed was performedon the renal biopsy of both groups. ELISA method and Western blot were used to determinethe expression of α1-antitrypsin and fibrinolytic enzyme-9 protein.RT-PCR was used to detect the mRNA expression of α1-antitrypsin and matrix metalloprotein-9. Results Blood urea nitrogen, serum creatinine,blood urea and uric acid of nephrotic syndrome patients were higher in patients at proteinuria stage（P 〈0.05）; α1-antitrypsin and matrix metalloprotein-9 detected by ELISA, Western blot and renal biopsy immunohistochemistry also showed that these indicators of nephrotic syndrome patients compared with those of urinary protein phase patientswere significantly reduced（P 〈0.05）. RT-PCR results showed that α1-antitrypsin and matrix metalloprotein-9 mRNA expression in nephrotic syndrome patients was also lower than that of patients at proteinuria stage. Conclusion α1-antitrypsin and proteolytic enzyme-9 are closely correlated with the progress of AL type renal amyloidosis and in the future can be used as a therapeutic target.
出处
《热带医学杂志》
CAS
2015年第3期355-358,F0004,共5页
Journal of Tropical Medicine
