摘要
目的:探讨高糖状态对体外培养血管内皮细胞迁移、增殖能力的影响,以及其潜在可能的分子机理。方法:通过不同质量分数的葡萄糖干预血管内皮细胞后,用CCK-8评估内皮细胞增殖情况;用transwell实验检测内皮细胞的迁移能力;用Real-time PCR检测腺苷酸环化酶(adenylate cyclase,AC)mRNA的表达;用Western blotting检测PKA、pPKA蛋白的表达情况。结果:高质量分数葡萄糖干预血管内皮细胞后,血管内皮细胞迁移、增殖能力明显下降,同时AC mRNA表达明显下降,PKA蛋白表达无明显变化,而p-PKA蛋白表达明显下降。结论:高质量分数葡萄糖使血管内皮细胞迁移、增殖能力下降,c AMP-PKA信号通路的活化可能参与了血管内皮细胞增殖、迁移及修复功能。
Objective：To determine the influence and its molecular mechanism of endothelial cell migration and proliferation in high glucos concentrations.Methods：The endothelial cells were treated in different glucose concentrations;and evaluated cell proliferation with CCK-8 reagent;and evaluated endothelial cell migration with transwell;and detected adenylate cyclase （ AC adenylate cyclase ） mRNA expression with fluorescence quantitative PCR;and detected PKA、p-PKA expression with Western blotting.Results：After endothelial cells were treated in high glucose concentrations , endothelial cell migration and proliferation significantly decreased ,and AC mRNA expression decreased obviously ,PKA of protein expression was not obviously changed ,and p-PKA expression decreased obviously.Conclusion：After endothelial cells were treated in high glucose concentrations , endothelial cell migration and proliferation are significantly decreased ,and cAMP-PKA activation may be involved in endothelial cell proliferation ,migration and repair functions.
出处
《现代临床医学》
2015年第2期117-120,共4页
Journal of Modern Clinical Medicine
基金
国家自然科学基金(81170081)
关键词
腺苷酸环化酶
环磷酸腺苷
蛋白激酶A
内皮细胞
血管损伤
adenylate cyclase,AC
cyclic adenosine 3’,5’ -monophosphate,cAMP
protein kinase A
endothelium cells
vascular injury
