摘要
目的探讨钙结合蛋白S100A12在小鼠重症急性胰腺炎治疗中的潜在作用。方法采用连续7次腹腔注射雨蛙素50μg/kg(每次间隔1 h)及脂多糖建立小鼠急性胰腺炎模型。将160只SPF级雌性ICR小鼠随机分为对照组(A组,生理盐水)、轻型组(B组,雨蛙素)、重症组(C组,雨蛙素+脂多糖)、干预组(D组,S100A12重组抗体+雨蛙素+脂多糖),每组40只。在首次注射雨蛙素后8 h、12 h和24 h采血,分别检测各时段血清中S100A12、淀粉酶(AMY)、C反应蛋白(CRP)、白介素(IL-1β、IL-6)、肿瘤坏死因子(TNF-α)的含量,观察小鼠胰腺的病理形态并进行评分。结果与C组相比,D组各时段血清中AMY、CRP、IL-1β、IL-6、TNF-α、S100A12含量明显降低(P<0.05);与B组相比,D组各时段血清S100A12浓度明显降低(P<0.05),胰腺病理形态也有明显改善。结论 S100A12重组抗体能够有效降低小鼠急性胰腺炎的严重程度,S100A12可以作为重症急性胰腺炎治疗的一个有效靶点。
Objective To investigate the potential effects of calcium binding protein S100A12 on curative effect of severe acute pancreatitis induced by caerulein and lipopolysaccharide in mice. Methods Intraperitoneal injection of 50 μg/kg caerulein for seven times（every interval time was an hour） and intraperitoneal injection of10 mg/kg lipopolysaccharide for once were applied to establish acute pancreatitis mice models. 160 cases of specific pathogen free ICR female mice were randomly divided into control group（group A, normal saline）, mild group（group B, caerulein）, severe group（group C, caerulein＋lipopolysaccharide） and intervention group（group D,S100A12 recombinant antibodies＋caerulein＋lipopolysaccharide）, each group had 40 cases. The blood was sampled at 8 hours, 12 hours, and 24 hours after the beginning of building animal models. In each period of time, we respectively detected the serum S100A12, amylase（AMY）, C-reactive protein（CRP）, interleukin（IL-1β, IL-6）and tumor necrosis factor（TNF-α） levels. In addition, we observed and scored the pancreas histopathology of the mice. Results Compared with group C, serum AMY, CRP, IL-1β, IL-6, TNF-α, S100A12 levels of group D in each same period of time were significantly decreased（P〈0.05）. Compared with group B, serum S100A12 concentration of group D in each same period of time was significantly decreased（P〈0.05）, and the pancreas histopathology was also much improved. Conclusion S100A12 recombinant antibodies are able to significantly reduce the severity of acute pancreatitis in mice. S100A12 may serve as a valid target of treatment in severe acute pancreatitis.
出处
《肝胆胰外科杂志》
CAS
2015年第2期111-115,共5页
Journal of Hepatopancreatobiliary Surgery
基金
浙江省科技厅资助项目(2013C33224)
