摘要
根据地衣芽孢杆菌的gyrB基因序列设计一对引物,扩增片段大小为507bp的基因片段,该方法对地衣芽孢杆菌DNA的扩增结果为阳性,对照菌株扩增结果均为阴性,对地衣芽孢杆菌检测的灵敏性为1pg总DNA量,成功建立了地衣芽孢杆菌PCR检测方法,该方法具有快速、灵敏度高、特异性强等优点,为地衣芽孢杆菌的分离及鉴定奠定了良好的基础。
In this study, based on gyrB gene sequences from Bacillus licheniformis, a pair of specific primers was designed. The size of the amplified product were 507 bp. The amplified results from Bacillus DNA were positive, but the results were negative from control strain. The sensitivity for Bacillus licheniformis was 1pg DNA. A PCR method for detection of Bacillus licheniformis was established. The method had the advantages of high-efficiency,high-sensitivity and high-specificity, and which set a good foundation for isolating Bacillus licheniformis from a complex microbial community samples.
出处
《饲料博览》
2015年第3期29-31,共3页
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基金
山东省现代农业产业技术体系生猪产业创新团队项目(SDAIT-06-011-14)
