摘要
目的观察丙戊酸钠对阿霉素体外抗乳腺癌Hs578T细胞作用的影响。方法 Western blot检测缝隙连接蛋白Cx43的表达;MTT法检测阿霉素的细胞毒性;Annexin V/PI双染法观察阿霉素对细胞早期凋亡的影响;Hochest 33258荧光染色法检测阿霉素对细胞晚期凋亡的影响。结果 Western blot结果显示丙戊酸钠可以显著增强缝隙连接蛋白Cx43的表达(P<0.01);MTT结果表明在细胞间缝隙连接功能形成的条件下,丙戊酸钠联用阿霉素组的细胞存活率显著低于阿霉素组(P<0.01);Annexin V/PI双染法显示丙戊酸钠联用阿霉素组的细胞早期凋亡率显著高于阿霉素组(P<0.01);Hochest 33258荧光染色结果显示丙戊酸钠联用阿霉素组的细胞晚期凋亡率显著高于单用阿霉素组(P<0.01)。结论丙戊酸钠可显著增强阿霉素的细胞毒性及其诱导的细胞凋亡作用,其机制可能与增强缝隙连接通讯功能有关。
Objective To investigate the effect of sodium valproate, a histone deacetylase inhibitor, on the cytotoxicity of doxorubicin in breast cancer cells. Methods Western blotting was used to assess Cx43 protein expression in breast cancer Hs578 T cells exposed to doxorubicin and sodium valproate. MTT assay was used to determine the cytotoxicity of doxorubicin;annexin V/PI double staining and Hochest 33258 fluorescence staining were employed to detect doxorubicin- induced early and late apoptosis, respectively. Results Western blotting showed that sodium valproate significantly increased Cx43 protein expression in Hs578 T cells（P〈0.01）. The cells exposed to both sodium valproate and doxorubicin showed significantly lowered cell viability compared with the cells exposed to doxorubicin alone（P〈0.01）. Exposure to both sodium valproate and doxorubicin resulted in significantly increased early and late cell apoptosis rate compared with doxorubicin treatment alone（P〈0.01）. Conclusion sodium valproate can significantly enhance the cytotoxicity of doxorubicin and increase doxorubicininduced apoptosis in breast cancer cells in vitro possibly by enhancing the gap junction function.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2015年第1期62-65,共4页
Journal of Southern Medical University
基金
国家自然科学基金(81001457)~~
关键词
乳腺癌
丙戊酸钠
阿霉素
凋亡
breast cancer
sodium valproate
doxorubicin
apoptosis
