摘要
目的探讨何首乌饮对β-淀粉样蛋白(Aβ)诱导的大鼠原代培养海马神经元损伤保护作用及可能机制。方法采用新生24h内的SD大鼠进行原代培养海马神经元,将培养10d的海马神经元分为模型组、何首乌饮保护组、何首乌饮治疗组、何首乌饮对照组及空白对照组。Hoechst33258染色观察海马神经元的凋亡率;免疫组织化学、Western blotting和RT-PCR检测各组海马神经元硫氧还蛋白1(Trx1)蛋白和mRNA的表达情况。结果模型组细胞凋亡率增高(P<0.05),Trx1蛋白和mRNA的表达量明显减少(P<0.05);与模型组相比,何首乌饮治疗组和预防组的凋亡率明显降低(P<0.05),Trx1蛋白和mRNA的表达量明显增加(P<0.05)。结论何首乌饮可明显减轻Aβ诱导的海马神经元的损伤,其机制可能与增加抗氧化蛋白Trx1的表达有关。
Objective To investigate the protective effect of Heshouwuyin on primary hippocampal neuronal cells induced by β-amyloid protein（ Aβ） and its possible mechanisms. Methods Hippocampal neurons collected were from newborn SD rats aged in 24 hours. Hippocampal cells which were cultured 10 days were divided into five groups： Model group,Heshouwuyin prevention group,Heshouwuyin treatment group,Heshouwuyin control group and Normal control group.Hoechst33258 staining was used to observe the apoptosis rate of the hippocampal cells. Immunohistochemistry staining was used to observe the expression of Trx1. The mRNA expression of Trx1 was detected in the hippocampal neuronal cells of each group with reverse transcription polymerase chain reaction,and the protein expression of Trx1 was detected in the hippocampal neuronal cells with Western blotting. Results The apoptosis rate of hippocampal neuronal cells was significantly increased in the Model group（ P〈0. 05）. In the Model group,the expresstion of Trx1 was significantly decreased（ P〈0. 05）.Compared with the Model group,the apoptosis rate of hippocampal neuronal cells was significantly decreased in the Heshouwuyin prevention group and Heshouwuyin treatment group（ P〈0. 05）. The mRNA and protein expresstions of Trx1 were significantly higher in Heshouwuyin prevention group and Heshouwuyin treatment group than that in the Model group（ P〈0. 05）. Conclusion Heshouwuyin can significantly alleviate the Hippocampal neurons injury induced by Aβ. The neuroprotective activities of Heshouwuyin can be related to its ability to increase the Trx1 expression level.
出处
《解剖学报》
CAS
CSCD
北大核心
2015年第2期175-181,共7页
Acta Anatomica Sinica
基金
国家自然科学基金(81373187)
河北省自然科学基金资助项目(H2013201139)
