摘要
以抗和不抗烟嘧磺隆的沈糯509R和沈糯509S为试材,通过向水培液添加烟嘧磺隆的方法,研究抗系与不抗系的幼苗外观表现和生理指标变化。结果表明:处理后两个近等基因系均比对照发生明显变化;各项指标随处理浓度的增加而反应加剧;抗系0.02 m L·L-1烟嘧磺隆培养液中甚至无明显反应,而不抗系反应强烈直至死亡;抗系与不抗系对烟嘧磺隆反应的快慢和变化趋势存在明显差异。试验证明,采用0.02 m L·L-1烟嘧磺隆水培液对自交系进行烟嘧磺隆抗性鉴定,无论以形态指标还是以生理指标都可得到准确的鉴定结果。
To evaluate the morphological and physiological index variations between seedlings exhibiting sensitive and resistant capabilities to nicosulfuron ,near - isogenic lines of waxy corns ,shennuo 509R and shennuo 509S ,were employed for analyses using hydroponic method with the addition of nicosulfuron .The results indicated that these two near- isogenic lines both were significantly different from the control .All responding indexes became intensified with the in-crease of nicosulfuron concentrations .The resistant line did not have a clear reaction to 0 .02 mL·L - 1 nicosulfuron , whereas the sensitive line showed a strong response ,which became even dead .The sensitive and resistant lines were found to display significant variations in sensitivities and levels to nicosulfuron .In conclusion ,the utilization of hydro-ponic method with 0 .02 mL·L - 1 nicosulfuron provided an accurate evaluation of inbred lines in responses to nicosulfuron on the basis of their morphological and physiological indexes .
出处
《干旱地区农业研究》
CSCD
北大核心
2015年第2期87-90,共4页
Agricultural Research in the Arid Areas
基金
辽宁省科技计划项目(2011201018)
"十二五"国家科技支撑计划项目(2011BAD16B12
2012BAD04B00)