摘要
目的探讨长链非编码RNA肺腺癌转移相关转录本1(MALAT1)在结肠癌组织中的表达及其临床意义。方法收集121例结肠腺癌组织标本,并以其自身正常组织作为对照,提取组织RNA,实时定量聚合酶链反应法检测结肠癌及其自身正常对照组织中MALAT1的表达水平,并在结肠癌细胞系SW620中过表达MALAT1,观察其对肿瘤细胞迁移的影响。结果结肠癌组织MALAT1表达较自身正常对照组织表达显著升高(P<0.01)。有淋巴结转移的结肠癌组织中MALAT1的表达高于无淋巴结转移的结肠癌组织(P<0.05);有淋巴结转移的结肠癌标本中,淋巴结组织中MALAT1的相对表达量高于结肠癌组织(P<0.05)。SW620转染pc DNA3/MALAT1基因组迁移细胞数明显高于单纯SW620细胞组和SW620转染载体pc DNA3组(P<0.05);单纯SW620细胞组与SW620转染载体pc DNA3组迁移细胞数比较差异无统计学意义(P>0.05)。结论在结肠癌中,长链非编码RNA-MALAT1可作为一个潜在的检测指标,为临床判定预后和后期治疗提供理论基础。
Objective To investigate the expression of long non-cording RNA metastasis associated lung adenocarcinoma transcript 1（ MALAT1） in colorectal cancer（ CRC） and its clinical significance. Methods Case-matched tissues of CRC tissue and adjacent normal tissue（ n = 121） were selected. Quantitative polymerase chain reaction was carried out to examine the expression of MALAT1 in CRC tissue and adjacent normal tissue. The MALAT1 was over-expression in SW620 cell line in order to observe its effect on the tumor cell migration. Results The expression of MALAT1 in CRC tissue was significantly higher than that in adjacent normal tissue（ P 0. 01）. The expression of MALAT1 in CRC tissue of the patients who had lymphatic metastasis was significantly higher than that of the non-lymphatic metastasis patients（ P 0. 05）. Similarly,in the CRC metastasis patients,there was a higher expression of MALAT1 in the nodes than that in the original tumor tissues（ P 0. 05）.The number of metastasis SW620 cells was higher in the pc DNA3 / MALAT1 gene group compared with SW620 group and pc DNA3-SW620 group（ P 0. 05）. While the number of metastasis SW620 cells had no significant difference between SW620 group and pc DNA3-SW620 group（ P 0. 05）. Conclusion The expression of MALAT1 has the potential to serve as a diagnostic indicator,it can provide the rationale for judging prognosis and later stage treatment of CRC.
出处
《新乡医学院学报》
CAS
2015年第4期329-331,共3页
Journal of Xinxiang Medical University
关键词
长链非编码RNA
肺腺癌转移相关转录本1
结肠癌
long non-cording RNA
metastasis associated lung adenocarcinoma transcript 1
colorectal cancer
