摘要
目的:分析趋化因子CXCL12及其受体CXCR4在人类精子的表达。方法:收集有生育史且精液常规分析结果正常男性精液标本10例,经过密度梯度离心纯化处理后,采用RT-PCR和免疫荧光化学染色技术在基因和蛋白水平分析CXCL12/CXCR4在人精子的表达。结果:虽然正常人精子同时表达CXCL12(0.641±0.180)与CXCR4(0.464±0.100)mRNA,但免疫荧光染色分析发现,在蛋白水平人精子主要表达CXCR4而不表达CXCL12,且CXCR4染色主要位于精子顶体后区。结论:CXCL12/CXCR4可能也是调节精子功能的重要分子。
Objective： To explore the expression patterns of the chemokine CXCL12 and its receptor CXCR4 in human sperm.Methods： We collected semen samples from 10 fertile men,performed density gradient centrifugation,and then determined the expressions of both CXCL12 and CXCR4 in the sperm by RT-PCR and immunofluorescence staining. Results： RT-PCR revealed the mRNA expressions of CXCL12（ 0. 641 ± 0. 180） and CXCR4（ 0. 464 ± 0. 100） in the sperm. However,only CXCR4 rather than CXCL12 was expressed at the protein level,and the positive staining for CXCR4 was observed mainly in the posterior part of the acrosome.Conclusion： CXCL12 and CXCR4 are involved as important molecules in regulating the function of human sperm.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2015年第3期225-228,共4页
National Journal of Andrology
基金
国家自然科学基金(81471511
81270753)
北京市卫生系统高层次卫生技术人才培养计划(2013-3-021)
首都医科大学基础与临床合作课题(13JL11)~~
