摘要
目的研究FHIT及P16基因在口腔正常黏膜组织、口腔黏膜下纤维性变(OSF)以及OSF癌变组织中的甲基化情况,探讨FHIT和P16基因甲基化在OSF癌变过程中的可能作用。方法收集经临床和病理确诊的正常口腔黏膜组织30例、OSF组织28例、OSF癌变组织30例。应用甲基化特异性PCR(MSP),检测各组样本中FHIT基因和P16基因启动子区Cp G岛的甲基化情况。结果口腔正常黏膜组织、OSF及OSF癌变组织中FHIT基因甲基化阳性率分别为0.00%、25.00%和40.00%,P16基因甲基化阳性率分别为0.00%、35.71%和53.33%。OSF组及OSF癌变组显著高于正常对照组(P<0.01);OSF癌变组显著高于OSF组(P<0.01)。结论FHIT和P16基因甲基化可能在OSF的癌变过程中起重要作用。
【Objective】To investigate aberrant Cp G island methylation of fragile histidine triad（FHIT） and p16 gene promoter and to elucidate the role of gene methylation in the malignant transformation of oral submucous fibrosis（OSF）.【Methods】The data of patients, who had been diagnosed as OSF（28 cases）, carcinogenesis of OSF（30 cases） and normal oral tissues（30 cases） in the Pathological Department, were collected. Methylation specific PCR（MSP） was used to detect Cp G island methylation status of the p16 and FHIT gene in OSF, cancerous OSF tissues and normal tissues.【Results】The frequencies of the FHIT methylation in the normal tissues, the OSF tissues and the cancerous OSF tissues were 0.00%, 25.00% and 40.00%, respectively, and those of the p16 methylation were 0.00%, 35.71% and 53.33%, respectively. The methylation frequencies of both genes in the OSF tissues and the cancerous OSF tissues were significantly higher than those in the normal tissues（P 〈 0.01）. The methylation frequencies of both genes in the cancerous OSF tissues were significantly higher than that in the OSF tissues（P 〈0.01）. 【Conclusions】Methylation of FHIT gene and p16 gene may play critical roles in malignant transformation of OSF.
出处
《中国现代医学杂志》
CAS
北大核心
2015年第9期10-13,共4页
China Journal of Modern Medicine
基金
湖南省财政厅基金项目(湘财教指[2014]98号)
