摘要
目的 与青霉素、利福平、万古霉素、替考拉宁等4种常用抗菌药物对比研究,分析磷霉素对肠球菌的接种效应.方法 选择2010年1月至2012年1月自中国医科大学附属第一医院住院患者分离的91株肠球菌,均为不重复菌株,包括粪肠球菌44株,屎肠球菌47株.采用琼脂稀释法测定磷霉素、青霉素、利福平、万古霉素、替考拉宁5种抗菌药物对91株肠球菌在低接种浓度[1×10^5菌落形成单位（cfu）/mL]和高接种浓度（1×10^7 cfu/mL）下的最低抑菌浓度（MIC）.结果 对于所有受试菌株,磷霉素在高接种浓度、低接种浓度下的MIC值均波动在16～64 μg/mL之间.受试菌株,不管是粪肠球菌,还是屎肠球菌,均对磷霉素的敏感率最高（100.0％）,耐药率最低（0）.磷霉素、青霉素、利福平、万古霉素、替考拉宁5种抗菌药物随着菌株接种浓度的增加,其菌株MIC值均升高不足8倍,即均不存在接种效应.结论 对于肠球菌引起的感染,由于磷霉素体外药物敏感试验显示其敏感性高,且不存在接种效应,因此可用于临床治疗.
Objective To investigate the inoculum effect of fosfomycin on enterococci,in comparison with penicillin,rifampin,vancomycin and teicoplanin.Methods A total of 91 strains of enterococci were obtained from the patients hospitalized in The First Affiliated Hospital of China Medical University from January 2010 to January 2012.There was no repeated strain.The 91 strains consisted of 44 Enterococcus faecalis and 47 Enterococcus faecium strains.Agar dilution method was used to measure the minimum inhibitory concentrations （MIC） of the five antibiotics against the 91 enterococci strains under standard low inoculation concentration （1 × 10^5 colony-forming units [cfu]/mL） and high inoculation concentration （1 × 10^7 cfu/mL）.Results For all tested strains,MIC of fosfomycin fluctuated between 16-64 tμg/mL in high and low inoculated concentrations.Among the tested strains,both Enterococcus faecalis and Enterococcus faecium had the highest drug-sensitive rate （100.0%） and the lowest drugresistance rate （0） to fosfomycin.With the increase of the inoculation concentration,MIC of the five antibiotics increased less than 8 times,which indicated that there was no inoculation effect.Conclusion Fosfomycin shows high drug-sensitivity against enterococci without inoculum effect in vitro,and it is recommended for the treatment of enterococci-infected patients.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2015年第2期87-90,共4页
Chinese Journal of Infectious Diseases
关键词
肠球菌
接种效应
磷霉素
Enterococcus
Inoculum effect
Fosfomycin
