摘要
为考察浸提条件对油蒿酸性物质的影响和探讨油蒿酸性物质的最佳提取方法,采用单因素试验和正交试验相结合的方法,研究不同浸提条件下油蒿根、茎、叶、种子中的酸性物质的含量。结果表明:最佳提取条件为,根:48 h、1:5、40℃;茎:48 h、1:40、40℃;叶:72 h、1:50、40℃;种子:72 h、1:5、40℃。按对结果的影响大小依次为,根:固液比、浸提温度、浸提时间;茎和叶:浸提温度、固液比、浸提时间;种子:固液比、浸提时间、浸提温度。在最佳处理条件下,所检测的油蒿根、茎、叶和种子中酸性物质的含量高于其他处理。油蒿不同部位检测到的主要酸性物质不同,其提取受浸提时间、浸提温度、固液比以及自身分解速度等多个因素的共同影响。
The paper aims to investigate the effect of extraction conditions on acidic substances of Artemisia ordosica and optimal extraction method, the experiment, combining single factor experiment and orthogonaldesign of 3 factors at 3 levels, was conducted to study acidic substances contents in roots, stems, leaves andseeds of Artemisia ordosica under different conditions. The results showed that the optimal conditions forextraction were 48 h, 1：5, and 40℃ for roots; 48 h, 1：40, and 40℃ for stems; 72 h, 1：50, 40℃ for leaves; 72 h,1：5 and 40℃ for seeds. The sequence according to the effect of the extraction results was as follows： solid/liquid ratio, extraction temperature, extraction time for roots, extraction temperature, solid/liquid ratio,extraction time for stems and leaves, solid/liquid ratio, extraction time, extraction temperature for seeds.Contents of detected acidic substances under optimal conditions for extraction were higher than that under theother treatments. The main acidic substances detected in different parts of Artemisia ordosica were differentand were affected by multiple factors, such as extraction time, extraction temperature, solid/liquid ratio andtheir decomposition speed, etc.
出处
《中国农学通报》
2015年第7期33-38,共6页
Chinese Agricultural Science Bulletin
基金
"十二五"国家科技支撑计划(2012BAD16B02)
高等学校博士学科点专项科研基金(200900141100/4)
国家自然科学基金资助项目"沙冬青叶组织细胞跨膜离子流对昆虫取食的初始响应机理"(30871727)
关键词
油蒿
浸提条件
酸性物质
Artemisia ordosica
extraction conditions
acidic substances
