摘要
为了研究不同类型土壤真菌对转几丁质酶基因烟草防御酶和养分的影响,通过盆栽试验,以转几丁质酶基因烟草(T-Chit)为供试材料,野生型烟草(Nt-X)为对照,分别接种大丽轮枝菌(Verticillium dahliae Kleb)和木霉(Trichodermasp),结果表明:在不接种处理(CK)中,T-Chit的苯丙氨酸解氨酶(PAL)活性显著高于Nt-X,但两者过氧化物酶(POD)活性及养分含量均无显著性差异.在木霉处理(T)中,T-Chit的PAL和POD活性较不接种处理分别提高89.1%和113.5%,此时T-Chit中两种防御酶活性均显著高于Nt-X,分别高出31.9%和25.3%,TChit根系全K含量比Nt-X显著降低了14.7%.在大丽轮枝菌处理(V)中,T-Chit的PAL和POD活性较不接种处理分别提高66.9%和69.4%,此时两种基因型烟草间PAL,POD活性均无显著性差异.另外,T-Chit根系全P含量比Nt-X低17.2%,且差异显著.总的来说,真菌侵染时,能有效诱导转几丁质酶基因烟草的防御性酶活性增强,而对转基因植株生长及营养状况基本无影响.
A pot experiment was conducted to study the effects of different types of fungi on defensive enzymes and nutritional status of chitinase-transgenic（McChit1）tobacco.The chitinase-transgenic tobacco（T-Chit）was taken as the experimental plant and the non-transgenic tobacco（Nt-X）as the control.Verticillium dahliae Kleb,apathogenic fungus,or Trichodermasp,a bio-control fungus,was inoculated into the rhizosphere of the plants.The results showed that when no inoculation（CK）was performed,the PAL activity of T-Chit was significantly higher than that of Nt-X but no marked differences in POD activity and nutritional status were found between them.With the inoculation of Trichodermasp（T）,the PAL and POD activities of T-Chit increased by 89.1%and 113.5%,respectively,compared with the non-inoculated plants,and the activities of both enzymes were significantly higher than those of Nt-X,the increase being 31.9%and 25.3%,respectively.In addition,the content of total K in the root system of T-Chit sharply decreased by 14.7%,compared with Nt-K.With the inoculation of V.dahliae（V）,the activities of PAL and POD increased by 66.9%and 69.4%,respectively,compared with the non-inoculated plants,while no differences were observed in PAL and POD activities between the two genotypes of tobacco.Besides,the content of total P in the root system of T-Chit was 17.2%lower than that of Nt-X,the difference being significant statistically.In general,in response to Trichodermasp and V.dahliae,the activities of plant defensive enzymes of T-Chit were distinctly enhanced,but the growth and nutrient content of the chitinase-transgenic tobacco were basically consistent with those of non-transgenic tobacco.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第4期81-88,共8页
Journal of Southwest University(Natural Science Edition)
基金
国家自然科学基金资助项目(30771388)
教育部高校博士学科点专项科研基金资助项目(20100182110008)
关键词
生防真菌
病原真菌
转几丁质酶基因烟草
防御酶
养分
bio-control fungus
pathogenic fungus
chitinase-transgenic（McChit1）tobacco
defensive enzyme
nutrient
