摘要
采用PCR方法,对分离于大菱鲆的30株致病性鳗弧菌进行了溶血素vah1基因、鞭毛蛋白flaA基因、金属蛋白酶empA基因及外膜蛋白ompU基因的检测,选取3个代表菌株的相应基因序列通过Blast与GenBank中公布的参考菌株进行核苷酸同源性比较分析。结果表明,所检测的30株鳗弧菌均携带vah1、flaA、empA、ompU基因,携带率为100%;所测代表菌株的vah1、flaA、empA基因同参考菌株间均具有较高的同源性(93.9%～99.9%),而外膜蛋白ompU基因,3株代表菌间存在较大差异(86.0%～95.9%),其中的S010610-1株与其他2株代表菌及参考菌株均存在较大差异,在进化树中单独聚为一支。3株代表菌的其他基因(vah1、flaA、empA)均具有较高的同源性(94.2%～99.5%),但均存在一定的差异性。
Thirty pathogenicity Vibrio anguillarum strains isolated from turbot （Scophthalmus maximus ）were investigated for the presence of virulence genes including hemolysin（yah1）, flagellin（JlaA） ,metalloprotease（ernpA） and outer membrane protein（ornpU） using PCR methods in this study, and the homology of virulence genes from diverse representative V. anguillarum strains were compared with available sequences in the NCBI GenBank. The results showed that all the detected strains contained the vahl,flaA, ernpA and ornpU genes, the carrier rates were 100%. The vahl,jqaA and empA genes of three V. anguillarum strains were sequenced, sharing 93.9%-99.9o/oo nucleotide sequence homology with the reference strains. There is big difference （86. 0^-95. 9~） of ornpU gene in the three representative strains, the ompU gene of strain S010610-1 has significant difference with the sequences of reference strains in the NCBI GenBank and the other representative strains,and form a separated cluster in the phylogenetic tree. There are higher homology （ 94. 2 %-99. 5 %） among other genes （vahl,flaA, ern pA） of representative strains, but all exist some difference.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2014年第8期1267-1271,1277,共6页
Chinese Journal of Veterinary Science
基金
河北省重点基础研究资助项目(11960503D)
现代农业产业技术体系河北省创新团队建设资助项目(岗位名称:特色海产品疫病防控与质量安全)
关键词
大菱鲆
鳗弧菌
毒力基因
序列分析
turbot
Vibrio anguillarurn
virulence gene
sequence analysis
