摘要
采用DNA条形码技术,对珠海出入境检验检疫局从南非进口的鲜橙和鲜葡萄中截获的3头卷蛾幼虫进行分子鉴定,采用通用引物LCO1490/HCO2198对其线粒体COI基因的部分序列进行PCR扩增和测序,并与Gen Bank数据库和DNA条形码数据库BOLDSYSTEMS v3中的已知序列比对,结果与Gen Bank中20条苹果异胫小卷蛾的序列同源性达99%以上,与BOLDSYSTEMS v3中46条苹果异胫小卷蛾的序列同源性达99%。结合幼虫的形态特征,综合判定该幼虫为苹果异胫小卷蛾Thaumatotibia leucotreta。
The technique of DNA barcoding was applied to identify three tortricid larvae intercepted by Zhuhai Entry-Exit Inspection and Quarantine Bureau in fresh orange and grape from South Africa. Using the primer LCO1490/HCO2198, the partial mitochondrial DNA of COI gene was sequenced and blasted with released data in Gen Bank and BOLDSYSTEMS v3 database, the results showed that the sequences of intercepted specimens were more than 99% indentified with 20 sequences of Thaumatotibia leucotreta in Gen Bank and 46 sequences of T.leucotreta in BOLDSYSTEMS v3. The larvae characters were also described in this paper. According to morphological characters and sequence, the larvae was identified as false codling moth T. leucotreta.
出处
《广东农业科学》
CAS
2015年第4期113-116,F0003,共5页
Guangdong Agricultural Sciences
基金
国家科技支撑计划项目(2012BAK11B03)
国家质检总局科技计划项目(2009IK277)
关键词
苹果异胫小卷蛾
DNA条形码
幼虫
分子鉴定
Thaumatotibia leucotreta
DNA barcode
larvae
molecular identification
