摘要
目的:本文探讨体外冲击波碎石术损伤肾脏的氧化应激机制研究及牛磺酸的改善作用。方法:对本院80例肾结石行体外冲击波碎石术患者进行分析。所有患者随机分为两组:牛磺酸组于体外冲击波碎石术前1d口服牛磺酸粉,安慰剂组服用安慰剂,维持7d(2g/次,tid)。分析两组术后微量蛋白尿、β2-微球蛋白、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)水平变化。Western Blotting法分析NOX2、NOX4及肿瘤坏死因子-α(TNF-α)的表达变化。以健康体检者为对照组。结果:与对照组相比,冲击波碎石后微量蛋白尿、β2-微球蛋白、MDA水平明显增高SOD及GSH-px水平和Bcl2明显降低,而NOX2/4、Bax及TNF-α的蛋白表达明显增强。牛磺酸组的上述指标得到部分恢复。结论:牛磺酸通过抑制氧化应激反应和细胞凋亡减弱体外冲击波碎石术对肾脏的损伤作用。
Objective; To explore the involvement of oxidative stress in kidney injury induced by extracorporeal shock wave lithotripsy and alleviations of taurine. Methods： 80 cases of patients received extracorporeal shock wave lithotripsy were divided into 2 groups： placebo group and taurine group. The microalbuminuria, β2-microglohulin as well as MDA and GSH-px were detected. The expression o{ NOX2, NOX4 and TNF-α were detected hy Western Blotting. Results.. Compared with con- trol group, the microalbuminuria, 132-microglobulin and MDA content were increased greatly while GSH-px was decreased dra- matically. At the same time, the expression of NOX2, NOX4, Bax and TNF-α was up-regulated greatly while Bel2 was down- regulated significantly. Taurine can alleviate these abnormalities greatly. Conclusion： Taurine recovers the kidney injury by in- hibiting over activated oxidative stress and apoptosis.
出处
《海南医学院学报》
CAS
2015年第5期654-656,共3页
Journal of Hainan Medical University
基金
东莞市科技计划资助项目(2011Z028)~~
关键词
体外冲击波碎石术
氧化应激
牛磺酸
肾脏损伤
Extracorporeal shock wave lithotripsy
Oxidative stress
Taurine
Kidney injury
