摘要
目的比较采用生理盐水、去离子水、自来水作为样本稀释液对免疫层析粪便隐血试验结果的影响,明确隐血试验最适稀释液,提高隐血试验的准确率。方法采集正常人抗凝全血,用3种稀释液对其倍比稀释后进行隐血检测。通过肉眼观测及Image J图像处理软件灰度分析,比较三者差异。连续2周收集临床粪便标本,潜血检测使用3种稀释液同时测定,计算阳性率差异。结果全血检测生理盐水组灰度明显高于其他2组,P<0.05;自来水组和去离子水组相比差异无统计学意义,P>0.05;生理盐水/去离子水灰度比为1.78±0.85;生理盐水/自来水灰度比为2.12±0.72;自来水/去离子水灰度比为0.80±0.05。结论生理盐水是粪便隐血试验的最适稀释液,相比其他稀释液,具有较高的阳性检出率。
Objective Comparison of normal saline,deionized water,tap water as the sample diluent for immunochromatographic fecal occult blood test to confirm optimum dilution for occult blood test and improve the accuracy of the occult blood test.Methods Acquisition normal anticoagulated whole blood and occult blood detection with three dilutes.Comparison of the test difference by visual observation and grayscale image processing software Image J.Fecal occult blood detection of consecutive weeks clinical specimens was measured using three dilutes,calculated the difference of positive rate.Results Grey level of saline group with whole blood detection was significantly higher than the other two groups,P 〈0.05,however deionized water and tap water group showed no statistically significant difference,P〉 0.05.Grey ratio of saline/deionized water was 1.78 ± 0.85,saline/tap water was 2.12 ± 0.72,tap water/deionized water was 0.80 ± 0.05.Conclusion Saline is the most suitable dilutions for fecal occult blood test with a high positive rate compared to other diluents.The results suggest that we should use saline diluent and avoid tap water diluent at work.
出处
《河南大学学报（医学版）》
CAS
2015年第1期40-42,共3页
Journal of Henan University:Medical Science
