摘要
Objective To elucidate the chemical components of Xueshuantong （XST） Lyophilized Powder and primarily disclose the chemical difference between XST and Panax notoginseng roots. Methods Liquid chromatography coupled with electrospray ionization tandem mass spectrometry （LC-ESI-MSn） was used to profile the saponins in XST and P. notoginseng. Structural elucidation was based on spectral analyses of negative and positive ESI-MS3 data, and the comparison of the retention behaviors. Results The optimized LC-MS profiling approach enabled resolution of major saponins. The negative mode ESI-MS3 fragmentation gave diagnostic information on the nature （neutral loss 162 Da for GIc, 146 Da for Rha, and 132 Da for a pentose） and sequence （priority： terminal 〉 inner） of sugars and sapogenins （m/z 475 for protopanaxatriol; re^z459 for protopanaxadiol）, while the intact glycosyl portion could be characterized by characteristic Zoα, Cnnα＋, and Cnβ＋ （n = 2 or 3） obtained in the positive mode. Ultimately, a total of 30 saponins were characterized from XST. Compared with the roots of P. notoginseng, three malonyl-ginsenosides, ginsenoside Rd, and gyponoside XVII （or its isomer） were almost undetectable, and showed potential significance for their differentiation. Conclusion The established LC-MS profiling approach is powerful for the chemical analysis of P. notoginseng and its preparations such as XST.
Objective To elucidate the chemical components of Xueshuantong （XST） Lyophilized Powder and primarily disclose the chemical difference between XST and Panax notoginseng roots. Methods Liquid chromatography coupled with electrospray ionization tandem mass spectrometry （LC-ESI-MSn） was used to profile the saponins in XST and P. notoginseng. Structural elucidation was based on spectral analyses of negative and positive ESI-MS3 data, and the comparison of the retention behaviors. Results The optimized LC-MS profiling approach enabled resolution of major saponins. The negative mode ESI-MS3 fragmentation gave diagnostic information on the nature （neutral loss 162 Da for GIc, 146 Da for Rha, and 132 Da for a pentose） and sequence （priority： terminal 〉 inner） of sugars and sapogenins （m/z 475 for protopanaxatriol; re^z459 for protopanaxadiol）, while the intact glycosyl portion could be characterized by characteristic Zoα, Cnnα＋, and Cnβ＋ （n = 2 or 3） obtained in the positive mode. Ultimately, a total of 30 saponins were characterized from XST. Compared with the roots of P. notoginseng, three malonyl-ginsenosides, ginsenoside Rd, and gyponoside XVII （or its isomer） were almost undetectable, and showed potential significance for their differentiation. Conclusion The established LC-MS profiling approach is powerful for the chemical analysis of P. notoginseng and its preparations such as XST.
基金
Twelfth Five-Year National Science&Technology Support Program(2012BA129B06)
