摘要
水稻甜菜碱醛脱氢酶2基因(Badh2)第7外显子和第2外显子的突变是导致稻米变香的主要原因。本研究分别对包含第7外显子或第2外显子的PCR产物进行测序分析,以明确27种适宜海南省种植的香稻品种的Badh2的突变序列。研究结果表明:海香5701、山栏香糯、海香6309等20个品种Badh2基因的突变类型为第7外显子发生了8 bp的缺失和3个碱基的突变,所有27个水稻香稻品种第2外显子序列都未发生改变。本研究结果可为这些香稻品种的保护与鉴定、及作为香味基因供体改良其他优良品种奠定基础。
Most of fragrant rice result from mutation in exon7 or exon2 of gene betaine-aldehyde dehydrogenase2（Badh2）.In this study,in order to explicit mutant sequence of Badh2 of 27 fragrant rice varieties which were grown in Hainan province,their PCR（polymerase chain reaction） products containing exon7 or exon2 were sequenced,respectively.The results showed that 8 bp-deletion in Badh2 exon7 was present in 20 varieties including Haixiang5701,Shanlanxingnuo and Haixiang6039 et al.,and mutation in exon2 was not observed in all of 27 varieties.This research laid foundation for the protection of identification of these fragrant rice varieties,and as a fragrance gene donor to improve other varieties.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第2期276-280,共5页
Molecular Plant Breeding
基金
教育部热带作物新品种选育工程中心与作物学重点学科联合资助项目(lhxm-2012-3)
海南大学中西部计划学科重点领域建设项目(ZXBJH-XK001)
海南省教育厅高校科研资助项目(Hjkj2012-12)
海南大学科研启动经费(KYQD1001)共同资助
