摘要
MicroRNA(miRNA)是一种内源性的单链非编码RNA,参与细胞的增殖凋亡分化和代谢等重要生理过程。近年来发现循环miRNA能够耐受RNA酶消化、强酸强碱、煮沸等极端条件,是一种潜在的疾病标志物。因此,miRNA的检测对疾病的早期诊断具有重要的生物学意义。电化学生物传感器由于其简易、便携、灵敏、反应快速以及价格低廉等特点而被研究者广泛应用于mi RNA检测的研究。本文综述了miRNA的电化学传感器的研究进展,评述了各类方法的优缺点,并对mi RNA电化学检测的发展趋势进行了展望。
MicroRNAs （miRNAs） are a class of short endogenous non-coding RNAs that involve in diverse biological processes of cells, including development, differentiation, proliferation, and apoptosis. In recent years, accumulating studies have shown that the circulating miRNAs are resistant to RNase digestion, extreme pH and temperature, and have been proposed as novel biomarkers in diagnostics. The detection of miRNA is of great biological significance for clinical diagnosis. Owing to the fact that electrochemical detector is simple, portable, sensitive, fast response and low cost, electrochemical biosensors have been recognized to be the most promising way for miRNA detection. In this paper, the progress of miRNA electrochemical biosensor is summarized. And the advantages and the disadvantages of all kinds of methods and prospect the miRNA electrochemical detection trends are reviewed.
出处
《分子诊断与治疗杂志》
2015年第2期114-121,共8页
Journal of Molecular Diagnostics and Therapy
基金
国家自然科学基金(81371901)
高等学校博士学科点专项科研基金(20134433110010)
广东省科技计划国际合作项目(2012B050600021)
