摘要
Acid hydrolysis mechanisms of orientin-2＂-O-galactopyranoside（OGA）,orientin and other flavone C-glycosides in the trollflowers[Trollius chinensis Bunge） were studied in this report for the first time.Hydrolysis parameters including temperature,acidity,solvent and reaction time were comprehensively investigated.OGA could be hydrolyzed to orientin,followed by an isomerization to isoorientin via a reversible Wessely-Moser rearrangement reaction under stronger acidic conditions.A first-order kinetic model fitted the hydrolysis process of OGA well.Under the optimal hydrolysis conditions of 80 ℃,1.0 mol/L H^＋ and 7 h reaction time,about 77%OGA was transformed to orientin with no detectable isoorientin.These results could be helpful for better understanding of the acid hydrolysis kinetics of flavone C-glycosides,as well as the preparation of these valuable components under controlled acid hydrolysis conditions.
Acid hydrolysis mechanisms of orientin-2＂-O-galactopyranoside（OGA）,orientin and other flavone C-glycosides in the trollflowers[Trollius chinensis Bunge） were studied in this report for the first time.Hydrolysis parameters including temperature,acidity,solvent and reaction time were comprehensively investigated.OGA could be hydrolyzed to orientin,followed by an isomerization to isoorientin via a reversible Wessely-Moser rearrangement reaction under stronger acidic conditions.A first-order kinetic model fitted the hydrolysis process of OGA well.Under the optimal hydrolysis conditions of 80 ℃,1.0 mol/L H^＋ and 7 h reaction time,about 77%OGA was transformed to orientin with no detectable isoorientin.These results could be helpful for better understanding of the acid hydrolysis kinetics of flavone C-glycosides,as well as the preparation of these valuable components under controlled acid hydrolysis conditions.
基金
supported by National Natural Science Foundation of China(No.81274190)
Jiangsu Provincial Science and Technology Commission(No.BE2012649)
Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
