摘要
目的观察α-SMA+的肿瘤相关成纤维细胞(cancer-associated fibroblasts,CAFs)与肺腺癌患者临床病理特征及肿瘤浸润免疫细胞的相关性,为认识CAFs在肺腺癌发生中的作用提供依据,并将为临床肺腺癌的诊疗提供新思路。方法收集56例临床手术切除且病理诊断为原发肺腺癌的肿瘤组织标本,收集患者一般情况及临床病理学特征等资料。免疫组织化学染色方法检测肺腺癌患者肿瘤标本中α-SMA、CD4、CD8及CD33的表达情况,病理学方法计数间质细胞及α-SMA阳性的CAFs,统计学方法分析CAFs比例与患者临床病理特征及肿瘤浸润免疫细胞的相关性。结果α-SMA+CAFs比例与患者吸烟及肿瘤TNM(tumor,node,metastasis)分期呈正相关(P<0.05),与肿瘤分化程度呈负相关(P<0.05),但与患者年龄、性别及肿瘤浸润CD4+T细胞、CD8+T细胞及MDSCs(myeloid-derived suppressor cells,MDSCs)的数量无明显相关。结论α-SMA+CAFs与肺腺癌的发生发展密切相关,其与肿瘤临床分期及分化程度的相关性提示其在肺腺癌的发生发展中起促进作用,对其深入机制的阐明将有助于完善肺腺癌的发生机制并将为其治疗提供新思路。
To understand the role of CAFs in the occurrence of lung adenocarcinoma, so as to develop a novel approach for diagnosis of lung adenocarcinoma, we investigated the distribution of α-SMA＋CAFs in lung adenocarcinoma and analyzed the correlation of CAFs with clinic pathological characteristics and tumor-infiltrated immune cells in patients. Tumor biopsies of 56 patients pathologically diagnosed as lung adenocarcinoma were collected and the expressions of α-SMA, CD4, CD8 and CD33 were immunohistochemically analyzed. The stromal cells and α-SMA＋CAFs were counted pathologically as well. The correlation between the ratio of CAFs and clinic pathological characteristics as well as tumor-infiltrated immune cells in patients was analyzed statistically. Data showed that the ratio of α-SMA＋CAFs was positively correlated with smoking status and TNM（tumor, node,metastasis） stages（P〈0.05）, however, negatively correlated with differentiation grades（P〈0.05）. But no significant correlation could be summarized in analyzing the age, gender, CD4＋and CD8＋tumor-infiltrated immune cells as well as MDSCs（myeloid-derived suppressor cells, MDSCs）. We concluded that CAFs play a critical role in the development of lung adenocarcinoma. Thus CAFs could be a new therapeutic target of lung adenocarcinoma.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2015年第3期230-233,239,共5页
Immunological Journal
