摘要
【目的】研究补喂支链氨基酸（BCAA）、肌酸（Cr）对伊犁马血浆抗氧化指标、激素水平、肌酸、葡萄糖浓度以及乳酸、尿酸和肌酐含量的影响,为支链氨基酸、肌酸作为伊犁马有效的营养添加剂提供参考数据。【方法】选取年龄3~5岁（平均4岁）,体重390~514 kg（平均457±50 kg）,经过良好训练的伊犁马公马12匹进行试验。分为3组,每组4匹,分别为对照组、试验Ⅰ组和试验Ⅱ组。对照组补喂不添加任何添加物的补喂料,试验Ⅰ组补喂添加BCAA的补喂料,试验Ⅱ组补喂添加Cr的补喂料。分别在补饲的0、1、14、21和31 d补喂后4 h颈静脉采集血液。采用试剂盒法测定相关指标。【结果】补喂Cr能够明显提高T-AOC能力,Cr补喂组与对照组差异显著（P〈0.05）,特别是在14和31d比对照组提高了21.79%和25.68%;补喂BCAA降低试验期间MDA的含量;对于血浆中的胰岛素、胰高血糖素和皮质醇等3种激素水平,肌酸、葡萄糖、乳酸、尿酸和肌酐的浓度与对照组相比差异不显著（P〉0.05）。【结论】补喂BCAA可以降低脂质过氧化产物MDA的浓度;补喂Cr可以提高SOD的活力和提高伊犁马血浆的T-AOC能力;补喂BCAA、Cr对伊犁马血浆中的胰岛素、胰高血糖素和皮质醇等3种激素的水平均无显著影响;对浆中肌酸、葡萄糖的浓度均无显著影响;对血浆中乳酸、尿酸和肌酐的含量均无显著影响。
【Objective】This study aims to determine the effects of the branched- chain supplemented with amino acid（ BCAA） and creatine（ Cr） on the change of the plasma antioxidant index,hormone level,the concentration of creatine,glucose,lactic acid,uric acid and creatinine in plasma of the Yili horses,thus providing reference data for effective nutritious additives in horse sports training fodder.【Method】12 well- trained male horses of 3- 5 years old（ average 4 years old）,weight 390- 514kg（ average 457 ± 50 kg） and the similar performance were selected as the subjects in the experiments. They were divided into 3 groups with four ineach and one group was fed with basal diet（ the control group）. The experimental group one was fed with diet supplemented with BCAA and the experimental group two was fed with diet supplemented with Cr. On the 0day,1 d,14 d,21 d and 31 d jugular vein blood was collected after feeding 4 hours later,and testing kit was used to measure the related indexes.【Result】The total antioxidant capacity in plasma was increased by the diet supplemented with creatine after feeding 4 h later,and the difference was significant compared with control group（ P 〈0. 05）. Especially on 14 d and 31 d it was increased by 21. 79% and 25. 68%; BCAA reduced MDA content during the test,No significant differences were observed in the concentrations of insulin,glucagon and cortisol hormones,creatine,glucose,lactic acid,uric acid and creatinine of plasma compared with the control group（ P〉0. 05）.【Conclusion】The results indicated that the diet supplemented with BCAA could reduce lipid peroxidation products MDA concentration; Fodder supplemented with Cr could improve SOD vigor and improve the Yili horse＇s serum total antioxidant capacity,but no obvious effects were found in other indexes.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2015年第2期325-333,共9页
Xinjiang Agricultural Sciences
基金
国家科技计划项目(2012BAD45B00)
新疆维吾尔自治区重大科技专项(201130101)
关键词
支链氨基酸
肌酸
伊犁马
抗氧化能力
branched-chain amino acid
creatine
Yili horse
antioxidant capacity
