摘要
目的:从人口腔龈上皮细胞中分选出P-钙黏蛋白(P-cadherin)阳性表达和阴性表达的细胞,并将之与人牙龈结合上皮细胞的黏附、增殖和迁移的生物学特性进行比较。方法:分离培养人口腔龈上皮细胞,通过磁珠从中分选出P-钙黏蛋白阳性表达和阴性表达的细胞;分离培养人牙龈结合上皮细胞;测定人牙龈结合上皮细胞、口腔龈上皮细胞及从中分选出的P-钙黏蛋白阳性表达和阴性表达细胞的黏附、增殖和迁移能力。结果:P-钙黏蛋白阳性表达细胞占全部口腔龈上皮细胞的20%。P-钙黏蛋白阳性表达的口腔龈上皮细胞和人牙龈结合上皮细胞表现出较强而相似的黏附和迁移能力,但前者增殖较旺盛(0.72±0.06),后者增殖较慢(0.60±0.05,P<0.05);而P-钙黏蛋白阴性表达的口腔龈上皮细胞与牙龈结合上皮相比,表现出的黏附(48%±6%vs.87%±11%,P<0.05)、增殖(0.36±0.04 vs.0.60±0.05,P<0.05)和迁移能力[(10.3±2.7)个vs.(23.4±4.8)个,P<0.05]均较弱。结论:P-钙黏蛋白阳性表达的口腔龈上皮细胞与牙龈结合上皮细胞在生物学特性方面有一定相似性而又有区别,提示口腔龈上皮细胞转化为牙龈结合上皮细胞的过程中,可能不仅仅是部分细胞的简单迁移,其中可能牵扯到更为复杂的基因、蛋白表达方面的改变。
Objective： To isolate P-cadherin positive and negative oral gingival epithelial cells,and to compare the biological characteristics with junctional epithelial cells. Methods： Human oral gingival epithelial cells and junctional epithelial cells were cultured. P-cadherin positive and negative cells were isolated from oral gingival epithelial cells. The cellular adhesion,proliferation and migration were measured and compared. Results： The P-cadherin positive cells accounted for 20% of oral gingival epithelial cells.Compared with juctional epithelial cells,P-cadherin positive oral gingival epithelial cells showed similar properties of adhesion and migration,and stronger proliferation ability（ 0. 72 ± 0. 06 vs. 0. 60 ± 0. 05,P〈 0.05）. P-cadherin negative oral gingival epithelial cells showed weaker ability of adhesion（ 48% ±6% vs. 87% ± 11%,P〈 0.05）,proliferation（ 0. 36 ± 0. 04 vs. 0. 60 ± 0. 05,P〈 0.05） and migration（ 10. 3 ± 2. 7 vs. 23. 4 ± 4. 8,P〈 0.05）. Conclusion： P-cadherin positive oral gingival epithelial cells showed some similar but different biological characteristics,compared with juctional epithelial cells,which suggested that during the process of transforming oral gingival epithelial cells into juctional epithelial cells,complex gene and protein changes were involved instead of simply cellular migration.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2015年第1期32-36,共5页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(81000442)
北京大学口腔医学院青年科研基金资助~~
关键词
牙龈
上皮细胞
细胞黏附
细胞增殖
细胞运动
Gingiva
Epithelial cells
Cell adhesion
Cell proliferation
Cell movement
