摘要
目的:本研究探讨血型抗原"剂量效应"现象对意外抗体鉴定结果判定的影响以及其出现的条件、范围、规律等。方法:选取利用微柱凝胶法明确鉴定出意外抗体格局的受血者标本共40例,使用AB型新鲜冰冻血浆作为介质,将受血者血浆稀释为不同的浓度梯度,选择谱细胞试剂中与受血者血浆意外抗体反应呈阳性的谱细胞,将其分为单剂量抗原谱细胞和双剂量抗原谱细胞,将前者分别与不同浓度梯度的受血者稀释后血浆进行反应(微柱凝胶法),选取出现阴性结果的血清最高稀释度来评价其与双剂量抗原阳性谱细胞反应的凝集强度,以此观察不同剂量的血型抗原与意外抗体反应时表现出的差异。结果:经过稀释的40例受血者血浆标本中,经微柱凝胶法检测后有31例出现了"剂量效应"现象,其中30例意外抗体与双剂量抗原谱细胞凝集强度≤2+,而与单剂量抗原谱细胞反应为阴性;1例意外抗体与双剂量抗原谱细胞凝集强度为3+,与单剂量抗原谱细胞反应为阴性;9例受血者血浆标本经稀释后与谱细胞反应均为阴性(此9例稀释前强度自1+-3+不等)。结论:Rh,MNS,Kidd,Duffy血型系统意外抗体与双剂量抗原谱细胞反应强度≤2+(微柱凝胶法)时,单剂量抗原反应通常会明显减弱甚至阴性,表现出"剂量效应"现象,进而可能干扰意外抗体鉴定。Rh,MNS,Kidd,Duffy血型系统的抗体易出现"剂量效应"现象。
Objective： This study was to investigate the influence of ＂dosage effect＂ on unexpected antibody identification and explore its condition, scope and regularity. Methods：A total of 40 blood recipient samples containing definite unexpected antibodies were selected by column agglutination technology, then AB fresh plasma was used to dilute the samples to obtain different concentrate liquid. After selecting panel cells which show positive with corresponding unexpected antibody in the serum, ＂single dosage＂ antigens were distinguished from ＂double dosage＂ ones, and then the antigen-antibody reactions were observed between ＂ single dosage＂ panel ceils and respective diluted recipient samples （by column agglutination technology）. It＇s believable that the highest concentration which retains a negative result was choose to evaluate the agglutination strength between ＂double dosage＂ panel cells and diluted unexpected antibody, and to observe the difference happened at different ＂dosage＂ antigens with unexpressed antibody. Results：Among 40 diluted recipient samples detected by column agglutination technology, the ＂dosage effect＂ appeared in 31 diluted samples. There were 30 samples in which the unexpected antibody agglutinated ＂double dosage＂ antigens ≤2 ＋ , while ＂ single dosage＂ antigens negative. It appeared in another 1 diluted sample, in which the unexpected antibody agglutinated ＂ double dosage＂ antigens 3 ＋. There were 9 diluted samples in which the unexpected antibody agglutinated panel cells showing negative results（ strength was between 1 ＋ -3 ＋ before dilution）. Conclusions： When the unexpected antibodies in Rh,MNS ,Kidd, Duffy agglutinated ＂ double dosage＂ antigens ≤2 ＋ （by column agglutination technology） , ＂single dosage＂ antibody reaction maybe weaken, even be negative, and it may cause the ＂dosage effect＂ to interfere the unexpected antibody identification . The ＂dosage effect＂ appears in Rh, MNS, Kidd, Duffy blood sy
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2015年第1期222-227,共6页
Journal of Experimental Hematology
关键词
剂量效应
意外抗体鉴定
双剂量抗原
单剂量抗原
＂dosage effect＂
unexpected antibody identification
＂double dosage＂ antigen
＂single dosage antigen
