摘要
以黄瓜耐冷材料长春密刺、冷敏材料北京截头为试材,利用人工气候箱进行低温(12℃/7℃,昼/夜)及恢复处理,研究低温胁迫下外源尸胺(Cad)对黄瓜幼苗电解质渗透率、丙二醛(MDA)含量、可溶性蛋白质含量、可溶性糖含量、脯氨酸含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性、抗坏血酸过氧化物酶(APX)活性及叶绿素荧光参数的影响。与对照相比,Cad处理可以使低温胁迫下长春密刺、北京截头的电解质渗透率分别降低18.61%、9.54%,MDA含量分别降低13.13%、12.54%,可溶性蛋白质含量分别增加16.99%、11.20%,可溶性糖含量分别提高9.35%、2.90%,脯氨酸含量分别上升20.54%、17.36%;使SOD、POD、CAT、APX的活性均显著上升,分别提高9.64%和8.42%、19.83%和18.97%、31.95%和41.29%、10.66%和17.13%;同时可改善幼苗叶绿素荧光参数的变化。在低温胁迫条件下,外源尸胺通过促进黄瓜幼苗叶片可溶性物质含量的增加及抗氧化酶活性水平的提高,来降低膜脂过氧化水平,从而增强黄瓜幼苗的抗冷性。
Cucumber（ Cucumis sativus L.） variety Changchun mici（ chilling-tolerant） and Beijing jietou（ chillingsensitive） were sprayed with cadaverine（ Cad） and were then subjected to low temperature of 12 ℃ /7 ℃（ day / night）and recovering treatment in the artificial climate chamber. The effects of Cad on the changes in electrolyte leakage rate,malondialdehyde（ MDA） content,soluble protein content,soluble sugar content,proline content,superoxide dismutase（ SOD） activity,peroxidase（ POD） activity and catalase（ CAT） activity,ascorbate peroxidase（ APX） activity and chlorophyll fluorescence parameters under low temperature stress in the seedling leaves were investigated. Compared with the control treatment（ water）,Cad pretreatment could significantly reduce electrolyte leakage of Changchun mici and Beijing jietou by 18. 61% and 9. 54% respectively and MDA contents by13. 13%,12. 54% respectively,whereas increase soluble protein contents by 16. 99% and 11. 20% respectively,soluble sugar contents by 9. 35% and 2. 90% respectively,proline contents by 20. 54% and 17. 36% respectively,and promote the activities of SOD,POD,CAT,and APX by 9. 64% and 8. 42%,19. 83% and 18. 97%,31. 95% and 41. 29%,and 10. 66% and 17. 13%,as well as improve the changes of chlorophyll fluorescence parameters in cucumber seedling leaves under low temperature stress. The application of exogenous Cad could improve chilling tolerance in cucumber seedlings by decreasing the membrane lipid peroxidation,increasing soluble substances content and enhancing the activities of antioxidant enzymes.
出处
《江苏农业学报》
CSCD
北大核心
2015年第1期122-129,共8页
Jiangsu Journal of Agricultural Sciences
基金
国家重点基础研究发展计划项目(2009CB119001-01
2012CB113900)
"863"计划专项(2010AA10A108
2012AA100202)
江苏省科技支撑计划项目(BE2009310)
江苏省农业科技自主创新基金项目[CX(11)1002]
关键词
黄瓜
外源尸胺
低温胁迫
生理生化指标
叶绿素荧光参数
cucumber
exogenous Cad
low temperature stress
physiological-biochemical index
chlorophyll fluorescence parameter
