摘要
目的：应用气相色谱-质谱（GC—MS）联用法对淫羊藿挥发油进行指纹图谱研究。方法：采用水蒸气蒸馏法提取巫山淫羊藿和粗毛淫羊藿中的挥发油，利用GC—MS对其化学成分进行分析，通过GC—FID建立了淫羊藿挥发油指纹图谱的分析方法。气相色谱条件为HP—INNOWAX色谱柱，进样口温度260℃，分流比5：1；程序升温（初始温度60℃，保持10min，以2℃·min^-1升至250℃，保持13min）。GC—MS参数为柱流速1．0mL·min^-1，MS电子能量70eV，离子源温度230℃，四级杆温度180℃，传输线温度280℃，全离子扫描，扫描质量范围m／z40～550，溶剂延迟3min。GC—FID参数：柱流速1．5mL·min^-1，FID检测器温度260℃，氢气流量30mL·min^-1，空气流量300mL·min^-1，尾吹氮气30mL·min^-1。结果：获得了20份不同产地淫羊藿的指纹图谱峰。结论：该方法的精密度、稳定性和重复性均良好。为淫羊藿药材的质量控制提供了有效手段。
Objective： To investigate the chromatographic fingerprints of volatile oil from Epimedii Folium by GC-MS. Method： The volatile oil was extracted from Epimedii Folium by steam distillation method, chromatographic fingerprints of which was detected and calculated by GC-FID, and the mutual fingerprints of ten kinds essential oil from Epimedii Folium was established. GC conditions： HP-INNOWAX column inlet temperature 260 ℃, the split ratio of 5：1 ; temperature program： initial temperature of 60 ℃ , keep 10 min, with 2 ℃·min^-1 rose to 250 ℃ , 13 min to maintain. GC-MS parameters： column flow rate 1.0 mL·min^-1, MS electron energy 70 eV, ion source temperature of 230 ℃ , the quadrupole temperature 180 ℃ , the transmission line temperature 280 ℃ , full-ion scan mass range m/z 40-550, solvent delay 3 min. GC-FID parameters： column flow rate 1.5 mL ·min^-1, FID detector temperature 260 ℃ , the hydrogen flow rate 30 mL·min^-1 , the air flow 300 mL ·min^-1, tail blowing nitrogen 30 mL ·min^-1 Result： The method was proved to be accuracy, reproducible and stability, by which the ideal fingerprints of volatile oil from Epimedii Folium were obtained. Conclusion： The methods set up are beneficial for the quality control of Epimedii Folium.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第7期39-42,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技支撑计划项目(2011BAI13B04-01)
2012年度教育部"创新团队发展计划"项目(IRT1227)
