摘要
以大花蕙兰与墨兰的杂交品种‘韩国小姐’为母本,墨兰品种‘企剑黑墨’为父本进行杂交,对其杂交坐果、落果情况及种子的无菌播种方法进行研究。结果表明:授粉后0~112d落果较为严重。150d时坐果率为62.1%。果荚采收时期及培养基对种子的无菌萌发影响显著,种子在授粉后194d采收,且于不添加植物生长调节剂的1/2MS培养基中培养时萌发最佳。
Cymbidium 'Miss Korea', the hybrid of Cymbidium hybridium and Cymbidium sinense, was crossed with Cymbidium sinense'Qijian Heimo' as male parent. The fruit set and drop and the aseptic seeding method of this cross combination were studied. The results showed that the fruit set was 62.1% on 150 days after pollination. The fruits dropped seriously during 0-112 days after pollination. Fruit harvesting time and nutrient media significantly influenced the germination frequency. Seeds, which harvested on 194 days after pollination, germinated best on half strength Murashige and Skoog(1/2MS) medium without plant growth regulators.
出处
《热带作物学报》
CSCD
北大核心
2015年第3期510-515,共6页
Chinese Journal of Tropical Crops
基金
厦门市科技计划项目(No.3502Z20142004)
福建省自然科学基金项目(No.2011J01097)
关键词
杂交兰
墨兰
杂交
坐果率
无菌播种
Hybrid
Cymbidium sinense
Interspecies cross
Furit set
Aseptic seeding