摘要
研究了加州鲈鱼（Micropterus salmoides）在10、20、30、40、50、60和70 mg/L的MS-222麻醉液中的麻醉效果,并在20 mg/L的MS-222麻醉液中研究了10、15、20和25℃水温对麻醉效果的影响,最后在20℃和20 mg/L的MS-222麻醉液中进行了麻醉模拟运输试验。麻醉试验结果表明：随着MS-222浓度的增加,进入相同麻醉期的时间缩短,完全复苏的时间延长。10-20 mg/L的MS-222适于加州鲈鱼的麻醉运输。麻醉时间随着水温的降低而缩短,复苏时间随着水温的降低而增加。20-25℃水温适于加州鲈鱼的麻醉运输。麻醉模拟运输试验结果表明：运输17 h存活率100%,运输24 h存活率降到70%。麻醉的鱼复苏时间随运输时间的增加而增加。麻醉运输后,肌肉中糖元含量下降,乳酸含量上升。血清皮质醇变化差异不显著但都显著高于基础组（P〈0.05）。天冬氨酸氨基转移酶（AST）、尿素（UREA）、乳酸脱氢酶（LDH）在运输前10 h差异不显著之后显著升高（P〈0.05）,葡萄糖（GLU）随运输时间的增加而增加。
This paper studied the anesthetic effects of MS-222 at the concentration of 10, 20, 30, 40, 50, 60 and 70 mg/L on largemouth bass （Micropterus salmoides） , then it studied the impact of water temperature at 10, 15, 20 and 25 ℃ in 20 mg/L MS-222 on anesthetic effects, and finally anesthesia simulated transportation was conducted at 20 ℃ and 20 mg/L MS-222. Anesthesia test results showed： With the rising of MS-222 concentration, anesthesia time shortened while full recovery time prolong. 10 -20 mg,/L MS-222 was suitable for largemouth bass anesthesia transportation. Anesthesia time shortened but recovery time increased with decreasing of water temperature. 20 - 25 ℃ water temperature was suitable for largemouth bass anesthesia transportation. Anesthesia simulated transportation test results showed： The survival rate was 100% after transportation 17h but it dropped to 70% after transportation 24h. Recovery time increased as transport duration increased. Muscle glycogen fell but lactic acid rose after anesthesia transportation. The differences of serum cortisol didn＇ t change significantly but were significantly higher than the initial group （P 〈 0.05 ）. There were no significant differences of aspartate transaminase （AST）, urea （UREA） and lactate dehydrogenase（LDH） in the first 10 h transportation, then they all increased significantly （P 〈 0.05 ）. Glucose （GLU） increased with transportation time.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2015年第2期235-241,共7页
Journal of Shanghai Ocean University
基金
"十二五"国家科技支撑计划(2012BAD38B00)
