摘要
目的：建立一种模拟脓毒症临床病理生理过程的动物模型。方法新西兰大白兔20只，随机分为模型组和对照组，每组10只。参见CASP方法改良制作脓毒症兔模型，对照组进腹后不进行肠穿孔，找到升结肠后关腹。CASP模型后4 h由右颈总动脉置管连接压力换能器，通过Medlab生物信号采集处理系统连续监测平均动脉压（MAP）、心率（HR）。分别于造模后4 h、8 h抽取颈动脉测定乳酸、血气、血糖、血浆TNF-α水平。术中监测呼吸频率及尿量，CASP后8 h开腹观察腹腔状况，检测肺组织病理学改变并行损伤评分。结果（1）模型组CASP后4～8 h MAP逐渐下降，HR逐渐增快，对照组MAP、HR变化平稳。（2）CASP后8 h时与对照组相比，模型组血乳酸、血糖水平增高，BE降低（P＜0.05）。（3）CASP后8 h时，与对照组相比，模型组SaO2、PaO2、PaCO2及pH值均下降。（4）CASP后4 h、8 h模型组TNF-α均高于对照组。（5）对照组肺损伤病理学评分低于模型组。结论运用改良CASP模型可成功复制出接近临床实际的腹腔感染诱发的脓毒症动物模型。
ObjectiveTo establish a reliable and stabilized animal model that mimics the clinical characteristics of sepsis.MethodsTwenty New Zealand White rabbits were randomly divided into 2 groups （n=10 each）： groupⅠsham operation （C）, groupⅡsepsis model （E）. Sepsis model was established according to modified colon ascendens stent peritonitis （CASP） in the study. Sham operation without puncturing the colonic wall were performed. The animals breathed spontaneously during the experiment. A catheters was introduced into the right carotid artery for blood sampling and connected to a pressure transducer for continuous mean arterial pressure （MAP） and heart rate （HR） monitoring. At 8 h after CASP, a median abdominal incision was made and photo documentation of abdominal situs was performed. Arterial blood samples were drawn at 4 h and 8 h after CASP for blood gas analysis, glucose and lactate. The plasma component was separated and tumor necrosis factor-alpha （TNF-α） were measured. Respiratory rate and urinary production were record. The rabbits were sacrificed at 8h after CASP. The sections of lung were stained with hematoxylin and eosin for light microscopic examinations and the injury score were record.Results（1）MAP declined gradually and HR tended to increase during the course of the experiment in group E, but there were no significant differences in group C. （2）Base excess （BE） were significantly lower but glucose and lactate Lac were higher in group E as compared with the group C at 8 h after CASP （P〈0.05）. （3） In group E, SaO2, PaO2, PaCO2 and pH decreased significantly at 8 h after CASP compared with group C （P〈0.05）. （4）TNF-α concentration in plasma was higher in group E than in group C at 4 h and 8 h after CASP （P〈0.05）. （5） In group E, morphological change scores of lung were significantly increased compared with group C （P〈0.05）.ConclusionThe modified CASP model can mimic closely the clinical course of diffuse peritonitis with early
出处
《中华临床医师杂志（电子版）》
CAS
2015年第5期76-79,共4页
Chinese Journal of Clinicians(Electronic Edition)
基金
安徽高校省级自然科学研究项目(KJ20088323)
皖南医学院重点科研项目培育基金(WK2014ZF03)
关键词
脓毒症
模型
动物
兔
Sepsis
Models, animal
Rabbit
