摘要
构建人白介素6(h IL-6)表位疫苗,研究其免疫原性。利用在线预测工具预测h IL-6线性B表位(Ala69-Asn80);重叠延伸PCR法对h IL-6进行基因融合,克隆到质粒p GEX-6P-1,并在大肠杆菌BL21(DE3)中表达;亲和层析法纯化融合蛋白,鉴定其纯度达95%以上;皮下免疫BALB/c小鼠,ELISA法测定抗血清效价,表位(Ala69-Asn80)移植到白喉毒素转膜区(DTT,Ser305-Ile310),构建的表位疫苗可刺激机体产生针对h IL-6的抗体,抗血清效价达1.0×105。
An interleukin-6 epitope vaccine is developed and its immunogenicity is investigated. Online B-cell epitope prediction tools were used to predict the potential linear B-cell epitope（ Ala69-Asn80）; The corresponding gene sequence of the epitope was fused with the DTT by over-lap PCR and the fused gene was cloned into expression vector p GEX-6P-1; Recombinant proteins were successfully expressed in Escherichia coli BL21（ DE3）; Recombinant proteins were purified by affinity chromatography and the purity reached to more than 95%; BALB / c mice was immunized by the purified and identified proteins subcutaneously and antiserum titer was determined by ELISA; The epitope vaccine transplanting h IL-6（ Ala69-Asn80） to Diphtheria toxin transmembrane domain（ DTT,Ser305-Ile310）could elicit strong immune response and antiserum titer reached to 1. 0 × 105.
出处
《济南大学学报（自然科学版）》
CAS
北大核心
2015年第1期34-38,共5页
Journal of University of Jinan(Science and Technology)
基金
上海市优秀学术/技术带头人计划项目(12XD1420500)
关键词
白介素6
B表位预测
免疫原性
表位疫苗
interleukin-6
B-cell epitope prediction
immunogenicity
epitope vaccine
