摘要
目的：建立HPLC-DAD测定通贤柚叶中柚皮苷与野漆树苷的方法。方法：色谱柱为Sepax Amethyst C18-H（250×4.60mm,5μm）,流动相为乙腈-0.1%甲酸梯度洗脱,流速1.0m L/min,检测波长为283nm（柚皮苷）,337nm（野漆树苷）,柱温25℃,进样量20μL。结果：柚皮苷与野漆树苷色谱峰分离良好。二者分别在0.0653~0.6526mg/m L与0.0350~0.3505mg/m L范围内与峰面积呈良好的线性关系。平均加样回收率分别为98.68%与98.47%。结论：所建立的含量测定方法可用于通贤柚叶的质量控制。采收季节与叶龄对柚叶中两种黄酮类物质的含量有较明显的影响。
Objective：To develop a HPLC-DAD method to determine the content of naringin and rhoifolin in Tongxian grapefruit leaves.Methods：HPLC was performed on a Sepax Amethyst C,8-H analytical column（25Omm × 4.60mm, 51μm）with gradient elution using acetonitrile and 0.1% aqueous formic acid as the mobile phase at a flow rate of 1.0mL/min. The detection wavelength was 283nm for naringin,337nm for rhoifolin.The column temperature was 25℃ and the injection volume was 20μL. Results： The chromatographic peaks of naringin and rhoifolin could be separated well in the above cases.The linear ranges for the two flavonoids were 0.0653-0.6526mg/mL and 0.0350- 0.3505mg/mL, respectively. The average recoveries were 98.68% and 98.47%, respectively.Conclusion： The established method could be used for the quality control of Tongxian grapefruit leaves.Harvest season and leaves age affect the content of two flavonoids significantly.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第7期286-289,共4页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31201946)
四川省教育厅科研项目(13ZC074)
四川省"高等教育质量工程"建设项目
