摘要
采用低氧通气的方法,对马铃薯富集γ-氨基丁酸(γ-aminobutyric acid,GABA)的培养工艺和培养液组分进行研究。首先通过正交实验优化了马铃薯富集GABA的工艺,接着采用Box-Behnken设计对影响马铃薯GABA富集的培养液组分进行了优化。结果表明,低氧通气富集马铃薯中GABA的最佳工艺为培养时间4h、培养温度20℃、培养液pH5.8。极差分析表明,培养液pH是最主要的影响因素,培养温度次之,最后是培养时间。在最适培养工艺下,马铃薯中GABA的富集量为0.3160mg/g,是原料中GABA含量的3.45倍;Box-Behnken设计优化的最优培养液组分为谷氨酸钠(MSG)浓度15.43mg/m L、CaCl2浓度2.81mmol/L和VB6浓度0.03mg/m L,在此条件下马铃薯中GABA含量为0.5749mg/g,是原料的6.28倍,说明优化后的培养液组分能显著提高马铃薯中GABA含量。方差分析表明,所建的回归模型显著,能很好地预测马铃薯中GABA含量的变化。
The culture processes and culture solution components of γ-aminobutyric acid （GABA）accumulation in potato using hypoxia aerating method were researched in this paper. Firstly, the culture processes of GABA accumulation in potato was optimized using orthogonal experimental design. Subsequently, the optimal culture solution components on GABA accumulation of potato were investigated using Box-Behnken design.The results showed that the optimum culture processes of GABA accumulation in potato under hypoxia aerating were culture time 4h,temperature 20~C, and pH of culture solutions 5.8.Range analysis indicated that pH of culture solutions was the most important influence factor, followed by culture temperature, and culture time was the last. Under these conditions,the accumulation of GABA in potato was 0.3160mg/g,which was 3.45 times of material.Box-Behnken experimental design results displayed the optimum compositions of culture solution were MSG concentration 15.43mg/mL,CaCI2 concentration 2.8lmmoVL, and VB6 concentration 0.03mg/mL Under these conditions, the highest GABA content of potato was 0.5749mg/g, 6.28- fold of material. It indicated that the optimized culture solution compositions significantly increased GABA content of potato. Analysis of variance indicated that the regression model was significant,could forecast the changes of GABA accumulation in potato excellently.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第7期241-245,251,共6页
Science and Technology of Food Industry
基金
江苏省高等学校大学生实践创新训练计划项目(2013)
关键词
马铃薯
Γ-氨基丁酸
富集
低氧通气
培养条件
potato
γ- aminobutyric acid
accumulation
hypoxia aerating
culture conditions
