摘要
目的 :通过体内实验研究β绒毛膜促性腺激素的第5号亚基(chorionic gonadotropin beta polpeptide 5,CGB5)对卵巢癌细胞OVCAR-3成瘤率及成瘤速度的影响。方法:采用慢病毒载体转染的方法 ,在卵巢癌细胞OVCAR-3中分别转入CGB5小干扰、过表达及无关序列。另外,未处理的OVCAR-3细胞作为空白对照,将高表达CGB5的绒癌细胞Be Wo作为阳性对照。用RT-PCR和酶联免疫吸附试验法测定转染后细胞的CGB5浓度。裸鼠随机分为5组(正常OVCAR-3细胞组,CGB5小干扰组,CGB5过表达组,CGB5无关序列组,Be Wo细胞组),每组6只,将这5种不同的细胞株分别打入裸鼠右侧腋窝皮下建立皮下移植瘤模型。观察5组模型的肿瘤成瘤率及成瘤速度,并用免疫组化测定肿瘤细胞增殖指标Ki67的表达情况。结果:(1)CGB5过表达组和Be Wo细胞组裸鼠的皮下移植瘤成瘤率是100%(6/6),OVCAR-3正常细胞组和无关序列对照组细胞的裸鼠的皮下移植瘤成瘤率是83.3%(5/6),而CGB5小干扰组细胞的裸鼠皮下移植瘤成瘤率是33.3%(2/6)。(2)CGB5过表达组和CGB5表达高的Be Wo细胞组的肿瘤生长较快,而CGB5小干扰组的肿瘤生长最慢。结论:CGB5能增加卵巢癌OVCAR-3细胞皮下移植瘤的成瘤率及肿瘤的生长速度,这将有望为卵巢癌的治疗提供新的思路。
Objective： To explore the effect of chorionic gonadotropin beta polpeptide 5（CGB5） on human epithelial ovarian cancer cell line OVCAR-3 in the tumor formation rates and growth speed in vivo. Methods： The CGB5 overexpressed vector,CGB5 targeted si RNA vector were constructed and transfected into OVCAR- 3（ empty vector as control）. The human choriocarcinoma Be Wo cell line with CGB5 high expressed was used as a positive control. After sorting by flow cytometry, and selected by Blasticidin, the stable vector expression cell lines were obtained. These cells（five groups： untreated OVCAR-3cells, CGB5 targeted si RNA transfected OVCAR-3 cells, target-off si RNA transfected OVCAR-3 cells, CGB5 overexpressed OVCAR-3 cells and Be Wo cells） were injected subcutaneously into the nude mice（6 mice/group）. Then the tumor formation rates and growth speed were examined. The expression of Ki67 was detected by the immunohistochemistry. Results： The tumor formation rates of nude mouse xenografts were 100%（6/6） for CGB5 overexpressed OVCAR-3 and Be Wo cells; 83.3%（5/6） for untreated or target-off si RNA transfected OVCAR-3 cells group; and 33.3%（2/6） for CGB5 knockdown OVCAR-3 cells. And the speeds of tumor growth were also different, Be Wo and CGB5 overexpressed OVCAR-3 cells were the growth fastest, but the CGB5 knockdown OVCAR-3 cells were the slowest. Conclusions： CGB5 may increase the tumor formation rates and growth speed, which may support a new way for ovarian cancer therapy.
出处
《南通大学学报（医学版）》
2015年第1期13-16,F0002,共5页
Journal of Nantong University(Medical sciences)
基金
国家自然科学基金青年基金资助项目(30801226)
