摘要
以离体保存的小叶龙竹试管丛芽为外植体进行脱分化形成愈伤组织和愈伤组织再分化的研究。结果如下：1丛芽、茎尖、茎节和叶片,都能长出愈伤组织,效果较好的是丛芽、茎尖,其次为茎节,叶片较差。2最适培养基为MS＋2,4-D 3 mg·L^-1＋Kt 0.2 mg·L^-1＋S 3%。3 1-3代愈伤组织分化芽的频率高,每隔6-8个月新启动一批丛芽诱导产生愈伤组织,可以保证高频稳定的愈伤组织再生系统的建立,以供后续的转基因研究。
The in vitro cultured clumpy buds of Dendrocalamus barbatus Hsuen et D. Z. Li were used to induce calli and regenerate plantlets from calli. The results showed that the calli could be induced from clumpy buds,shoot tips,internodes and leaves respectively,and the clumpy buds was the most effective. The optimum medium for calli induction was MS ＋ 2,4-D3. 0 mg·L^- 1＋ Kt 0. 2 mg·L^- 1＋ S 3%. A high frequency plant regeneration system for this species can be established by induced calli from new clumpy buds in every 6 to 8 months,which could be provided for transgenic research.
出处
《竹子研究汇刊》
北大核心
2014年第4期1-5,共5页
Journal of Bamboo Research
基金
中国西南野生生物种质资源库项目(0802261411)
国家和云南省联合基金(U1136603)
国家自然科学基金(31100178)
云南省政府创新团队计划(2009CI011)
关键词
小叶龙竹
丛芽
愈伤组织培养
植株再生
Dendrocalamus barbatus
Cluster buds
Callus culture
Plant regeneration
